Chemical modification of the complex of tRNA Phe with phenylalanyl-tRNA synthetase from Escherichia coli
Ankilova, V.N.; Vlasov, V.V.; Mamaev, S.V.; Nuzhdina, N.A.
Molekuliarnaia Biologiia 13(5): 994-1000
1979
ISSN/ISBN: 0026-8984
PMID: 388193
Accession: 039529641
Alkylation of E. coli tRNAPhe, bound to the cognate synthetase was investigated. The alkylating reagent is a derivative of 2-chloroethylamine: 2',3'-O-[4(N-2-chloroethyl-N-methylamino)-benzylidene]-uridine-5'-methylphosphate. It was found that the enzyme protects from the reaction D-stem (guanosine G24) and the region of juxtaposition of acceptor stem and D-stem (S4U8 and C13) in the tRNAPhe.
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