+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Cloning and expression analysis of two ZFY-related zinc finger genes from Alligator mississippiensis, a species with temperature-dependent sex determination

Cloning and expression analysis of two ZFY-related zinc finger genes from Alligator mississippiensis, a species with temperature-dependent sex determination

Gene 119(2): 221-228

ISSN/ISBN: 0378-1119

PMID: 1398103

DOI: 10.1016/0378-1119(92)90275-t

In order to investigate the molecular mechanism of temperature-dependent sex determination, a human zinc finger gene (ZFY), known to be highly conserved amongst other species, was used to isolate homologues from the genome of the American alligator, Alligator mississippiensis. ZFY was originally a candidate for the primary testis-determining gene in man, but is now thought to function further down the sex-determining cascade. Two alligator genes are described, Zfc and Znc6. Both code for zinc finger proteins and exhibit amino acid (aa) homologies to ZFY of 91% and 73%, respectively. Znc6 shows aa homology of 88% to the protein encoded by the zinc finger exon of the human ZFY-related gene, ZNF6, recently found on the X chromosome. Analysis of Zfc and Znc6 expression during embryonic development identified two major transcripts of 5.9 kb and 2.7 kb coding for Zfc, whilst only one transcript of 4.8 kb was detected for Znc6. Both genes are transcribed at all stages tested, from day 3 (post egg laying) throughout gestation. The expression level of all transcripts appears to decline towards the time of hatching (65-72 days). No sex-specific differences in the expression were observed. The extensive sequence conservation of the genes between reptiles and humans suggests major functional constraints. The expression patterns indicate that these genes do not play a primary role in temperature-dependent sex determination.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 039589877

Download citation: RISBibTeXText

Related references

Cloning and expression analysis of two zfy related zinc finger genes from the american alligator a species with temperature dependent sex determination. Journal of Cellular Biochemistry Suppl. (16 Part A): 95, 1992

Gonadal sex differentiation in Alligator mississippiensis, a species with temperature-dependent sex determination. Cell and Tissue Research 273(1): 149-162, 1993

RNA-seq analysis of the gonadal transcriptome during Alligator mississippiensis temperature-dependent sex determination and differentiation. Bmc Genomics 17: 77, 2016

Temperature-dependent sex determination in Alligator mississippiensis. Journal of Zoology 200: 3-77, 1983

The image of memory: relationship between autobiographical memory and mental imagery in Korsakoff syndrome. Applied Neuropsychology. Adult 2020: 1-7, 2020

The cloning and expression analysis of Lhx9 during gonadal sex differentiation in the red-eared slider turtle, Trachemys scripta, a species with temperature-dependent sex determination. Journal of Experimental Zoology. Part B Molecular and Developmental Evolution 320(4): 238-246, 2013

SRY-like genes and temperature dependent sex-determination in the American alligator. Journal of Cellular Biochemistry Suppl 1995(19B): 53, 1995

Expression analysis of rice A20/AN1-type zinc finger genes and characterization of ZFP177 that contributes to temperature stress tolerance. Gene 420(2): 135-144, 2008

Effect of temperature on central chemical control of ventilation in the alligator Alligator mississippiensis. Journal of Experimental Biology 179: 261-272, 1993

The effect of body temperature on ventilatory sensitivity to carbon di oxide in the alligator alligator mississippiensis. Physiologist 24(4): 129, 1981

Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: Zinc finger-mediated impairment of cell growth. Gene 174(2): 225-233, 1996

Effect of temperature on the rates of digestion amino acid absorption and assimilation in the alligator alligator mississippiensis. Comparative Biochemistry and Physiology A 83(3): 585-588, 1986

Sex-biased expression of sex-differentiating genes FOXL2 and FGF9 in American alligators, alligator Mississippiensis. Sexual Development: Genetics Molecular Biology Evolution Endocrinology Embryology and Pathology of Sex Determination and Differentiation 7(5): 253-260, 2013

Metabolic heating of embryos and sex determination in the American Alligator, Alligator mississippiensis. Journal of Thermal Biology 28(2): 159-165, 2003

Isolation and determination of the primary structure of a lectin protein from the serum of the American alligator (Alligator mississippiensis). Comparative Biochemistry and Physiology. Part B Biochemistry and Molecular Biology 161(2): 161-169, 2012