+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cloning and expression of the gene for a fibronectin-binding protein from Staphylococcus aureus

Cloning and expression of the gene for a fibronectin-binding protein from Staphylococcus aureus

Embo Journal 6(8): 2351-2357

The gene encoding the fibronectin-binding protein (FNBP) from Staphylococcus aureus strain 8325-4 was isolated from a gene bank in pBR322. The original clone, containing a 6.5-kb insert, gave a functional product present in the periplasm of Escherichia coli. Analysis of polypeptides isolated after affinity chromatography on fibronectin-Sepharose followed by ion-exchange chromatography revealed two gene products, 87 and 165 kd in mol. wt. The amino acid compositions of these two polypeptides and a native FNBP from S. aureus strain Newman were very similar. Antibodies raised against the native FNBP from strain Newman precipitated the 125I-labelled 165-kd polypeptide, and unlabeled 165- and 87-kd polypeptides as well as native FNBP inhibited the immunoprecipitation reactions. The region of the fnbp-gene encoding the fibronectin-binding activity has been identified and subcloned in an expression vector based on the staphylococcal protein A gene. The resulting product in E. coli is an extracellular fusion protein consisting of two IgG-binding domains of protein A followed by a fibronectin-binding region. The fusion protein binds to fibronectin and completely inhibits the binding of fibronectin to intact cells of S. aureus.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 039590192

Download citation: RISBibTeXText

PMID: 2822388

DOI: 10.1002/j.1460-2075.1987.tb02511.x

Related references

Cloning and expression of fibronectin-binding protein A gene of Staphylococcus aureus in Escherichia coli. Veterinary Science in China 36(4): 266-269, 2006

Impacts of sarA and agr in Staphylococcus aureus strain Newman on fibronectin-binding protein A gene expression and fibronectin adherence capacity in vitro and in experimental infective endocarditis. Infection and Immunity 72(3): 1832-1836, 2004

Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus. Febs Journal 277(21): 4490-4505, 2010

Molecular cloning and expression of the gene for elastin-binding protein (ebpS) in Staphylococcus aureus. Journal of Biological Chemistry 271(26): 15803-9, 1996

Impacts of sar and agr upon Staphylococcus aureus fibronectin-binding protein A gene activation and fibronectin adherence capacity in vitro and in experimental infective endocarditis. Abstracts of the General Meeting of the American Society for Microbiology 103: B-087, 2003

Cloning, expression, and nucleotide sequence of a Staphylococcus aureus gene (fbpA) encoding a fibrinogen-binding protein. Infection and Immunity 63(5): 1914-1920, 1995

Variance in fibronectin binding and fnb locus polymorphisms in Staphylococcus aureus: identification of antigenic variation in a fibronectin binding protein adhesin of the epidemic CMRSA-1 strain of methicillin-resistant S. aureus. Infection and Immunity 69(6): 3791-3799, 2001

Molecular Interactions of Human Plasminogen with Fibronectin-binding Protein B (FnBPB), a Fibrinogen/Fibronectin-binding Protein from Staphylococcus aureus. Journal of Biological Chemistry 291(35): 18148-18162, 2016

Identification of D motif epitopes in Staphylococcus aureus fibronectin-binding protein for the production of antibody inhibitors of fibronectin binding. Infection and Immunity 65(2): 537-543, 1997

IL-17A plays an important role in protection induced by vaccination with fibronectin-binding domain of fibronectin-binding protein A against Staphylococcus aureus infection. Medical Microbiology and Immunology 206(3): 225-234, 2017

The fibrinogen- and fibronectin-binding domains of Staphylococcus aureus fibronectin-binding protein A synergistically promote endothelial invasion and experimental endocarditis. Infection and Immunity 76(8): 3824-3831, 2008

Fibronectin-binding protein A of Staphylococcus aureus has multiple, substituting, binding regions that mediate adherence to fibronectin and invasion of endothelial cells. Cellular Microbiology 3(12): 839-851, 2001

Reassessing the role of Staphylococcus aureus clumping factor and fibronectin-binding protein by expression in Lactococcus lactis. Infection and Immunity 69(10): 6296-6302, 2001

Cloning and characterization of a gene for a 19 kDa fibrinogen-binding protein from Staphylococcus aureus. Molecular Microbiology 12(4): 599-606, 1994

The A domain of fibronectin binding protein B of Staphylococcus aureus contains a novel fibronectin binding site. 2011