Section 40
Chapter 39,592

Cloning of all EcoRI fragments from phage lambda in E.coli

Bernardi, A.; Bernardi, G.

Nature 264(5581): 89-90


ISSN/ISBN: 0028-0836
PMID: 794729
Accession: 039591159

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Cloning of Dna fragments is a new powerful technique in molecular genetics and a number of interesting experiments involving both prokaryotic and eukaryotic genomes have already been reported (see ref. 1 for review). We present here results concerning the cloning of all Eco Ri (ref. 2) fragments from phage λ Dna in Escherichia coli using as a vector plasmid p SC101 (ref. 3). To achieve this, we had to solve the problem of propagating the λ fragment containing the immunity region since the phage strain used had a temperature-sensitive mutation in that region.

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