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Clostridium perfringens iota toxin ADP-ribosylates skeletal muscle actin in Arg-177

Vandekerckhove, J.; Schering, B.; Bärmann, M.; Aktories, K.

Febs Letters 225(1-2): 48-52

1987


ISSN/ISBN: 0014-5793
PMID: 2891567
DOI: 10.1016/0014-5793(87)81129-8
Accession: 039592897

Clostridium perfringens iota toxin ADP-ribosylates actin. Substrates of C. perfringens toxin are both non-muscle beta/gamma-actin and skeletal muscle actin. This finding suggests that C. perfringens iota ADP-ribosylates the same amino acid in skeletal muscle and non-muscle actin as does C. botulinum C2 toxin in non-muscle actin. Protein chemical analysis involving thermolysin cleavage on [32P]ADP-ribosylated actin or tryptic digestion followed by a secondary thermolysin cleavage of the radiolabelled fragments showed one major site of ADP-ribosylation. From its amino acid composition and sequence, the radiolabelled peptide was identified as peptide 175-177, locating the acceptor ADP-ribosyl amino acid as Arg-177.

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