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Combined effect of human recombinant interferon alpha, beta and gamma on tumor cell growth and the cell cycle in vitro



Combined effect of human recombinant interferon alpha, beta and gamma on tumor cell growth and the cell cycle in vitro



Gan to Kagaku Ryoho. Cancer and ChemoTherapy 14(5 Pt 1): 1234-1239



Antiproliferative effect and the effect on cell cycle distribution of human recombinant interferon (rHu-IFN)-alpha, -beta and -gamma in combination on a human colon cancer cell line were investigated in vitro. Combined treatment with rHuIFN-beta and rHuIFN-gamma had a marked antiproliferative effect. Combination of rHuIFN-beta (200U/ml) and rHuIFN-gamma (200JRU/ml) had a similar antitumor effect to rHuIFN-gamma (5,000JRU/ml). From the analysis of the cell cycle obtained by flow cytometry, rHuIFN-beta showed accumulation of the S phase, while rHuIFN-gamma revealed accumulation of the G1 phase. With the combination of rHuIFN-beta and rHuIFN-gamma, G2 + M phase decreased markedly and G1 phase increased. These results suggest that combined treatment with rHuIFN-beta and rHuIFN-gamma can be an effective therapy for human cancer.

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Accession: 039606045

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PMID: 3107476


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