+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparative 31P and 1H NMR studies on rat astrocytes and C6 glioma cells in culture



Comparative 31P and 1H NMR studies on rat astrocytes and C6 glioma cells in culture



Biochimie 74(9-10): 919-930



Rat astroglial cells in primary culture (95% enrichment) and C6 glioma cells were adapted to grow on microcarrier beads. In vivo 31P NMR spectra were collected from cell-covered beads perfused in the NMR tube. The NMR-visible phosphorylated metabolite contents of both cell types were determined using saturation factors calculated from the values of longitudinal relaxation times determined for C6 cells using progressive saturation experiments. On the other hand, the amounts of phosphorylated metabolites in cells were determined from proton decoupled 31P NMR spectra of cell perchloric acid extracts. The results indicate that the NTP and Pi contents of the normal and tumoral cells were similar, whereas the PCr level was higher in C6 cells and the NDP and phosphomonoester levels higher in astrocytes. The comparison of 1H NMR spectra of cell perchloric acid extracts evidenced larger inositol and alanine contents in C6 cells, whereas larger taurine and choline (and choline derivatives) contents were found in astrocytes. The Glu/Gln ratio was very different, 3.5 and 1 in C6 cells and astrocytes, respectively. In both cases, the more intense resonance in the 1H NMR spectrum was assigned to glycine. Based on the comparison of the metabolite content of a tumoral and a normal cell of glial origin, this work emphasizes the usefulness of a multinuclear NMR study in characterizing intrinsic differences between normal and tumoral cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039614327

Download citation: RISBibTeXText

PMID: 1334701

DOI: 10.1016/0300-9084(92)90076-q


Related references

Comparative phosphorus-31 and proton NMR studies on rat astrocytes and C6 glioma cells in culture. Biochimie 74(9-10): 919-930, 1992

Comparative studies of phosphoinositide hydrolysis induced by endothelin-related peptides in cultured cerebellar astrocytes, C6-glioma and cerebellar granule cells. Biochemical and Biophysical Research Communications 168(2): 512-519, 1990

Differential radiation response between normal astrocytes and glioma cells revealed by comparative transcriptome analysis. OncoTargets and Therapy Volume 10: 5755-5764, 2017

Phosphatidylserine-dependent phagocytosis of apoptotic glioma cells by normal human microglia, astrocytes, and glioma cells. Neuro-Oncology 2(3): 174-183, 2000

Phosphatidylserine-dependent phagocytosis of apoptotic glioma cells bynormal human microglia, astrocytes, and glioma cells. Neuro-Oncology 2(3): 174-183, 2000

Tunneling nanotubes between rat primary astrocytes and C6 glioma cells alter proliferation potential of glioma cells. Neuroscience Bulletin 31(3): 371-378, 2015

Effects of phenobarbital and phenytoin on the growth morphology and some ATPase activities of rat brain astrocytes derived from normal newborn cerebrum and C6 glioma cells in culture. Zhonghua Yi Xue Za Zhi 42(6): 425-436, 1988

Glutamate/glutamine metabolism coupling between astrocytes and glioma cells: neuroprotection and inhibition of glioma growth. Biochemical and Biophysical Research Communications 450(1): 295-299, 2014

The spreading of human normal glial and malignant glioma cells in culture. Studies on standard culture conditions. Acta Pathologica Microbiologica et Immunologica Scandinavica. Section A Pathology 93(5): 235-249, 1985

Multinuclear NMR spectroscopy studies on NH4Cl-induced metabolic alterations and detoxification processes in primary astrocytes and glioma cells. Developmental Neuroscience 20(4-5): 417-426, 1998

The biological studies of human glioma cells in tissue culture. Annual Report of the Institute for Virus Research Kyoto University 11: 58, 1968

Scanning electron microscopy of malignant gliomas. A comparative study of glioma cells in smear preparations and in tissue culture. Acta Neuropathologica 41(3): 217-221, 1978

Comparative studies of the binding of dimeric and monomeric enkephalins to neuroblastoma glioma ng 104 15 cells. Journal of Neurochemistry 45(1): 101-107, 1985

Mechanically induced calcium movements in astrocytes, bovine aortic endothelial cells and C6 glioma cells. Journal of Membrane Biology 174(2): 121-134, 2000

Proteomic analysis of rat astrocytes and C6 glioma cells. Zhonghua Yi Xue Za Zhi 87(48): 3421-3424, 2007