+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparative EPR studies on the nitrite reductases from Escherichia coli and Wolinella succinogenes



Comparative EPR studies on the nitrite reductases from Escherichia coli and Wolinella succinogenes



Febs Letters 218(2): 227-230



Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with g values of 9.7, 3.7, 2.9, 2.3 and 1.5 were observed. These signals disappeared upon reduction by dithionite. Reaction of reduced enzyme with nitrite resulted in the formation of ferrous heme-NO complexes with distinct EPR spectral characteristics. The heme-NO complexes formed with the two enzymes differed, however, in g values and line-shapes. When reacted with hydroxylamine, reduced enzymes also showed the formation of ferrous heme-NO complexes. These results suggested the involvement of an enzyme-bound NO intermediate during the six-electron reduction of nitrite to ammonia catalyzed by these two hexaheme nitrite reductases. Heme proteins that can either expose bound NO to reduction or release it are significant components of both assimilatory and dissimilatory metabolisms of nitrate. The different ferrous heme-NO complexes detected for the two enzymes indicated, nevertheless, their subtle variation in heme reactivity during the reduction reaction.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039614341

Download citation: RISBibTeXText

PMID: 3036590

DOI: 10.1016/0014-5793(87)81051-7


Related references

Comparative epr studies on the nitrite reductases from escherichia coli and wolinella succinogenes. Abstracts Of The Annual Meeting Of The American Society For Microbiology: Stract K50, 1984

Comparison of the structural and kinetic properties of the cytochrome c nitrite reductases from Escherichia coli, Wolinella succinogenes, Sulfurospirillum deleyianum and Desulfovibrio desulfuricans. Biochemical Society Transactions 34(Pt 1): 143-145, 2006

Regulation of the hexaheme nitrite/nitric oxide reductase of Desulfovibrio desulfuricans, Wolinella succinogenes and Escherichia coli. A mass spectrometric study. Febs Letters 276(1-2): 67-70, 1990

Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb. nov., and Description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from Humans with Periodontal Disease. International Bulletin of Bacteriological Nomenclature and Taxonomy 31(4): 432-445, 1981

Kinetic studies on the nitrite reductase of Wolinella succinogenes. Biochemical Journal 233(2): 553-557, 1986

Spectroscopic studies of partially reduced forms of Wolinella succinogenes nitrite reductase. Febs Letters 264(2): 257-262, 1990

Wolinella new genus wolinella succinogenes vibrio succinogenes new combination and description of bacteroides gracilis new species wolinella recta new species campylobacter concisus new species and eikenella corrodens from humans with periodontal disease. International Journal of Systematic Bacteriology 31(4): 432-445, 1981

Comparative study of lipopolysaccharides from wolinella recta wolinella curva wolinella succinogenes and campylobacter sputorum ssp sputorum. FEMS Microbiology Letters 81(3): 291-298, 1991

Electron paramagnetic resonance and magnetic circular dichroism studies of a hexa-heme nitrite reductase from Wolinella succinogenes. Febs Letters 219(1): 244-248, 1987

Periplasmic sulphide dehydrogenase (sud) from Wolinella succinogenes: Isolation, nucleotide sequence of the sud gene and its expression in Escherichia coli. European Journal of Biochemistry 227(1-2): 137-142, 1995

Two structurally and kinetically distinct forms of Wolinella succinogenes nitrite reductase. Biochemical Journal 271(1): 259-264, 1990

The purification and some equilibrium properties of the nitrite reductase of the bacterium Wolinella succinogenes. Biochemical Journal 233(2): 547-552, 1986

The membranous nitrite reductase involved in the electron transport of wolinella succinogenes. Archives of Microbiology 140(4): 380-386, 1985

The oxidative and nitrosative stress defence network of Wolinella succinogenes: cytochrome c nitrite reductase mediates the stress response to nitrite, nitric oxide, hydroxylamine and hydrogen peroxide. Environmental Microbiology 13(9): 2478-2494, 2011

Nitrite reductases of escherichia coli. Journal of General Microbiology 68(3): XIII, 1971