+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparative activities of rabbit complements of different ages using an in-vitro marrow purging model

Comparative activities of rabbit complements of different ages using an in-vitro marrow purging model

Leukemia Research 14(5): 407-416

In order to determine the best time to obtain complement for use in marrow purging in vitro, we have used 51Cr-release and limiting dilution assays (LDA) to evaluate the ability of serum from rabbits 20-30 (25d), 30-40 (35d) and 40-50 (45d) days old to lyse a series of neoplastic target cells in the presence of complement activating antibodies. Utilizing a limiting dilution assay (LDA) to measure log depletion of tumor cells in a 20-fold excess of normal bone marrow, treatment with monoclonal antibody and 25d complement depleted at least 4 logs of leukemia or lymphoma cells. 35d and 45d complements were approximately one log less effective. When normal bone marrow alone was treated with either J5 (CD10) antibody alone, each complement alone or a combination of J5 antibody with each complement, there was no significant depletion of hematopoietic progenitors in any subgroup. These results suggest that complement from 20-30 day old rabbits should be used for the purpose of ex-vivo purging due to its highly sensitive and specific activity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 039614438

Download citation: RISBibTeXText

PMID: 2345466

DOI: 10.1016/0145-2126(90)90026-6

Related references

Comparative efficiency of an immunomagnetic purging procedure and a rabbit complement lysis to eliminate bl cells from the bone marrow. Mani, J C And J Dornand (Ed ) Lymphocyte Activation And Differentiation: Fundamental And Clinical Aspects; 18th International Leucocyte Culture Conference, La Grande Motte, France, June 19-24, 1987 Xxi+960p Walter De Gruyter And Co : Berlin, West Germany; Hawthorne, New York, Usa Illus 845-848, 1988

In vitro purging of bone marrow with mafosfamide synergizes with in vivo chemotherapy to delay the hematological recovery in a murine model of autologous bone marrow transplantation. Experimental Hematology 21(2): 311-318, 1993

Autologous bone marrow transplantation in acute myeloid leukemia after in-vitro purging with an anti-lacto-N-fucopentaose III antibody and rabbit complement. Leukemia Research 11(3): 265-272, 1987

Breast cancer and laser photoradiation therapy: an in vitro model for autologous bone marrow purging. Progress in Clinical and Biological Research 333: 103-107, 1990

Cost analysis of immunomagnetic marrow purging for neuroblastoma: in-house purging versus submission to purging centers. Journal of HematoTherapy 4(1): 41-44, 1995

Complement dependent antibody mediated cytolysis of myeloma cells in vitro a model for autologous bone marrow purging. Proceedings of the American Association for Cancer Research Annual Meeting 27: 326, 1986

Establishment and application of a murine transplant model of bone marrow purging of metastatic breast cancer cells in vitro. Zhonghua Xue Ye Xue Za Zhi 28(9): 621-623, 2007

Combination of hyperthermia with liquid marrow culture for purging leukemic marrow in vitro and application to autologous bone marrow transplantation in treatment of patients with acute leukemia. Experimental Hematology 22(8): 778, 1994

Long term bone marrow culture as a model system for in vitro purging of lymphoma cells by CD3XCD19 bispecific antibodies. Experimental Hematology 24(9): 1151, 1996

The incorporation of in vitro hyperthermia in the purging of leukemic bone marrow prior to autologous marrow transplantation abmt. Proceedings of the American Association for Cancer Research Annual Meeting 29: 360, 1988

Autologous bone marrow transplantation with microwave marrow purging in vitro for treatment of acute leukemia of 4 cases. Xi'an Yike Daxue Xuebao 17(1): 53, 1996

In vitro purging of bone marrow for autologous marrow transplantation in acute myelogenous leukemia using myeloid-specific monoclonal antibodies. Bone Marrow Transplantation 3(5): 387-392, 1988

Autologous bone marrow transplantation after purging by low molecular weight natural tumor suppressor in long-term marrow culture in vitro: report on 2 cases. Zhonghua Nei Ke Za Zhi 30(8): 495-7 522, 1991

Bone marrow purging with monoclonal antibodies plus complement: Usefulness of long-term bone marrow cultures as a control in vitro. Medical Science Research 24(9): 585-588, 1996

In vitro purging of clonogenic leukemic cells from human bone marrow by heat: Simulation experiments for autologous bone marrow transplantation. Leukemia Research 16(10): 973-977, 1992