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Comparative activity of arylsulphatases A and B on two synthetic substrates



Comparative activity of arylsulphatases A and B on two synthetic substrates



Biochemical Journal 157(2): 353-356



Rat liver and human skin fibroblasts arylsulphatase A and B activities on both 4-methylumbelliferyl sulphate and 4-nitrocatechol sulphate were compared. The intracellular distribution of activity differed markedly when 4-methylumbelliferyl sulphate was used from that observed with 4-nitrocatechol sulphate. No discrimination between control and metachromatic leucodystrophy or mucopolysaccharidosis (type VI) could be achieved when 4-methylumbelliferyl sulphate was used as substrate. These results contrast sharply with those obtained with 4-nitrocatechol sulphate and cast doubt on the validity of 4-methylumbelliferyl sulphate as substrate for the determination of arylsulphatase A and B activities.

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Accession: 039614466

Download citation: RISBibTeXText

PMID: 962873

DOI: 10.1042/bj1570353


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