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Comparative analysis of the structure of double-stranded RNA from killer strains of Saccharomyces cerevisiae

Comparative analysis of the structure of double-stranded RNA from killer strains of Saccharomyces cerevisiae

Molekuliarnaia Biologiia 22(1): 224-230

Double-stranded RNAs (M and L molecules) of two strains of the killer system Saccharomyces cerevisiae M437 (wild type) and ski-5 (superkiller mutant) were studied by means of electron microscopy and high resolution thermal melting. The M molecules of the ski-5 mutant were by 100 b.p. shorter than those of M437. L molecules were of the same length for both strains. Analysis of the differential melting curves of L molecules showed that L molecules differ significantly in their nucleotide sequences, whereas M molecules were practically identical. It was found that M molecules contained a long AU region: that of M molecules of M 437 was 170-180 b.p. long and contained almost no GC pairs, whereas the AU region of M molecules of the ski-5 mutant was three times shorter and contained GC pairs.

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Accession: 039614965

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PMID: 3287136

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