+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of the ability of basic and acidic fibroblast growth factor to stimulate the proliferation of an established keratinocyte cell line: modulation of their biological effects by heparin, transforming growth factor beta (TGF beta), and epidermal growth factor (EGF)



Comparison of the ability of basic and acidic fibroblast growth factor to stimulate the proliferation of an established keratinocyte cell line: modulation of their biological effects by heparin, transforming growth factor beta (TGF beta), and epidermal growth factor (EGF)



Journal of Cellular Physiology 142(2): 325-333



The bioactivity of both bFGF and aFGF in the BALB/MK-1 cell line has been compared to that of EGF. Our results indicate that, for that cell type, aFGF was far more potent than bFGF in inducing cell proliferation. In the presence of heparin, aFGF was as potent as EGF. In addition, excess bFGF has an inhibitory effect on the proliferation of MK cells exposed to a saturating concentration of aFGF, therefore acting as a partial agonist of aFGF. Surprisingly, bFGF, although it had low biological activity, was capable of synergizing the effect of EGF. In its presence, cultures exposed to saturating concentration of EGF have a final cell density 3- to 4-fold higher than that of counterpart cultures exposed to EGF alone. TGF beta, which in previous studies has been shown to inhibit the growth of keratinocytes, also inhibited the growth of BALB/MK-1 cells in response to either bFGF or aFGF. These studies suggest a role for FGF in regulating BALB/MK proliferation. aFGF provides positive growth signals which can be negatively modulated by excess bFGF or TGF beta, while bFGF, although a poor mitogen, could act by potentiating the effect of subsaturating concentrations of EGF.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039633969

Download citation: RISBibTeXText

PMID: 2303528

DOI: 10.1002/jcp.1041420215


Related references

Comparison of the ability of basic and acidic fibroblast growth factor to stimulate the proliferation of an established keratinocyte cell line: modulation of their biological effects by heparin, transforming growth factor b (TGFb), and epidermal growth factor (EGF). Journal of Cellular Physiology 142: 5-33, 1990

Growth factor regulation of the renal cortical tubular cells by epidermal growth factor, insulin-like growth factor-I, acidic and basic fibroblast growth factor, and transforming growth factor-beta in serum free culture. Cell Biology International Reports 13(8): 687-699, 1989

Epidermal growth factor, transforming growth factor alpha, transforming growth factor beta, acidic fibroblast growth factor, basic fibroblast growth factor, and interleukin-1 proteins in the cornea. Experimental Eye Research 59(1): 63-71, 1994

Chemotaxis of human keratocytes is increased by platelet-derived growth factor-BB, epidermal growth factor, transforming growth factor-alpha, acidic fibroblast growth factor, insulin-like growth factor-I, and transforming growth factor-beta. Current Eye Research 17(1): 79-87, 1998

Modulation of transforming growth factor-beta 1 stimulated elastin and collagen production and proliferation in porcine vascular smooth muscle cells and skin fibroblasts by basic fibroblast growth factor, transforming growth factor-a, and insulin-like growth factor-I. Journal of Cellular Physiology 155: 9-56, 1993

Modulation of transforming growth factor-beta 1 stimulated elastin and collagen production and proliferation in porcine vascular smooth muscle cells and skin fibroblasts by basic fibroblast growth factor, transforming growth factor-alpha, and insulin-like growth factor-I. Journal of Cellular Physiology 155(1): 149-156, 1993

Effects of epidermal growth factor egf basic fibroblast growth factor bfgf and transforming growth factor beta tgf beta on bovine corneal cell chemotaxis. Investigative Ophthalmology & Visual Science 32(4): 953, 1991

Differential effects of basic fibroblast growth factor, epidermal growth factor, transforming growth factor-alpha, and insulin-like growth factor-I on a hypothalamic gonadotropin-releasing hormone neuronal cell line. Journal of Neuroscience Research 49(6): 739-749, 1997

Effect of growth hormone, insulin-like growth factor I, basic fibroblast growth factor, and transforming growth factor beta on cell proliferation and proteoglycan synthesis by avian postembryonic growth plate chondrocytes. Journal of Bone and Mineral Research 9(3): 431-439, 1994

Modulation of the mitogenic response of an epidermal growth factor-dependent keratinocyte cell line by dexamethasone, insulin, and transforming growth factor-beta. Journal of Cellular Physiology 136(2): 257-265, 1988

Regulatory effects of cell density on the binding of transforming growth factor beta, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor. Cancer Research 48(15): 4266-4271, 1988

Receptors for transforming growth factor type beta tgf beta platelet derived growth factor pdgf fibroblast growth factor fgf and epidermal growth factor egf are regulated by cell density. Proceedings of the American Association for Cancer Research Annual Meeting 30: 72, 1989

The effect of epidermal growth factor basic fibroblast growth factor transforming growth factor beta and insulin on the dna synthesis of renal cell carcinoma cell lines. Acta Medica Nagasakiensia 36(1-4): 47-51, 1991

Expression of the angiogenic factors vascular endothelial cell growth factor, acidic and basic fibroblast growth factor, tumor growth factor beta-1, platelet-derived endothelial cell growth factor, placenta growth factor, and pleiotrophin in human primary breast cancer and its relation to angiogenesis. Cancer Research 57(5): 963-969, 1997

Synergistic action of angiotensin II, insulin-like growth factor-I, and transforming growth factor-beta on platelet-derived growth factor-BB, basic fibroblastic growth factor, and epidermal growth factor-induced DNA synthesis in vascular smooth muscle cells. American Journal of Hypertension 6(6 Pt 1): 496-499, 1993