EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Conformational aspects of beta-trypsin interaction with substrates and pancreatic trypsin inhibitor. III. Catalytic act of trypsin and its inhibition


Conformational aspects of beta-trypsin interaction with substrates and pancreatic trypsin inhibitor. III. Catalytic act of trypsin and its inhibition



Molekuliarnaia Biologiia 20(2): 357-368



ISSN/ISBN: 0026-8984

PMID: 3702867

Basing on the results of the theoretical conformational analysis of the nonbonded and valence complexes of trypsin with substrate molecules, the catalytical act of the enzyme is described in details as a spontaneous process. Conformational aspects of interactions of trypsin with pancreatic trypsin inhibitor are analysed. The complete inhibition process and the geometry of the enzyme-inhibitor complex are described in details. The point amino acid replacements, which will provide for an exclusion of BPTI inhibition and will radically change the specificity of the enzyme are proposed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039660206

Download citation: RISBibTeXText

Related references

Conformational aspects of interactions of beta trypsin with substrates and pancreatic trypsin in inhibitor iii. the catalytic act of trypsin and its bovine pancreatic trypsin inhibitor inhibition. Molekulyarnaya Biologiya (Moscow) 20(2): 357-368, 1986

Conformational aspects of beta-trypsin interaction with substrates and pancreatic trypsin inhibitor. I. Conformational properties of residues in the enzyme-active center and the structure of a non-valent enzyme-substrate complex. Molekuliarnaia Biologiia 20(1): 102-119, 1986

Conformational aspects of beta-trypsin interactions with substrates and pancreatic trypsin inhibitor. II. Structure of tetrahedral adducts and acylenzyme. Molekuliarnaia Biologiia 20(2): 346-356, 1986

Conformational aspects of interactions of beta trypsin with substrates and pancreatic trypsin inhibitor ii. structures of tetrahedral adducts and of acyl enzyme. Molekulyarnaya Biologiya (Moscow) 20(2): 346-356, 1986

Conformational aspects of interactions of beta trypsin with substrates and pancreatic trypsin inhibitor i. conformational properties of residues in enzyme active center and structure of nonbonded enzyme substrate complex. Molekulyarnaya Biologiya (Moscow) 20(1): 102-119, 1986

Hydrogen exchange kinetics of bovine pancreatic trypsin inhibitor beta-sheet protons in trypsin-bovine pancreatic trypsin inhibitor, trypsinogen-bovine pancreatic trypsin inhibitor, and trypsinogen-isoleucylvaline-bovine pancreatic trypsin inhibitor. Biochemistry 26(11): 3156-3167, 1987

Studies on the interaction of trasylol aprotinin with trypsin ec 3.4.21.4 pancreatic secretory trypsin inhibitor complexes and with alpha 2 macro globulin trypsin pancreatic secretory trypsin inhibitor complexes. Hoppe Seyler's Zeitschrift fuer Physiologische Chemie 362(12): 1653-1656, 1981

Interaction of alpha chymotrypsin or beta trypsin with basic pancreatic trypsin inhibitor. Experientia 32(6): 807, 1976

Hydrogen exchange kinetics of bovine pancreatic trypsin inhibitor β-sheet protons in trypsin-bovine pancreatic trypsin inhibitor, trypsinogen-bovine pancreatic trypsin inhibitor, and trypsinogen-isoleucylvaline-bovine pancreatic trypsin inhibitor. Biochemistry (Easton) 26(11): 3156-3162, 1987

Hydrogen exchange kinetics of bovine pancreatic trypsin inhibitor b-sheet protons in trypsin-bovine pancreatic trypsin inhibitor, trypsinogen-bovine pancreatic trypsin inhibitor, and trypsinogen-isoleucylvaline-bovine pancreatic trypsin inhibitor. Biochemistry (American Chemical Society) 26: 56-62, 1987

The nature of trypsin-pancreatic trypsin inhibitor binding: free energy calculation of Tyr39 Phe39 mutation in trypsin. Chemical Biology and Drug Design 50(5): 382-387, 1997

The nature of trypsin-pancreatic trypsin inhibitor binding: free energy calculation of Tyr39-->Phe39 mutation in trypsin. Journal of Peptide Research: Official Journal of the American Peptide Society 50(5): 382-387, 1997

Circular dichroism spectroscopy of bovine pancreatic trypsin inhibitor and five altered conformational states. Relationship of conformation and the refolding pathway of the trypsin inhibitor. Biochemistry 20(20): 5744-5754, 1981

On trypsin inhibitors iii modification and behavior of inhibition of the trypsin inhibitor from porcine pancreas a hydrolysis catalyzed at acid ph values enz trypsin. Z Physiol Chem 349(12): 1645-1656, 1968

Transient removal of proflavine inhibition of bovine beta-trypsin by the bovine basic pancreatic trypsin inhibitor (Kunitz). A case for "chronosteric effects". Journal of Biological Chemistry 258(8): 4676-4678, 1983