Section 40
Chapter 39,671

Construction of a hybrid col E1 plasmid carrying the gene for bacteriophage lambda repressor

Tikhomirova, L.P.; Solonin, A.S.; Ksenzenko, V.N.; Matvienko, N.I.

Nucleic Acids Research 3(10): 2485-2490


ISSN/ISBN: 0305-1048
PMID: 1069256
DOI: 10.1093/nar/3.10.2485
Accession: 039670589

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A biologically active hybrid DNA molecule was constructed from plasmid Col E1 and the Eco R1 fragment of lambda DNA containing the gene for lambda repressor. The presence of this gene in the hybrid molecule was demonstrated genetically. The hybrid plasmid contains two closely located targets for restriction endonuclease Hind 111 in the integrated fragment. Thus, the plasmid may be used as a vector not only for Eco R1 fragments but also for Hind 111 fragments.

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