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Contranscription of genes for RNA polymerase subunits beta and beta' with genes for ribosomal proteins in Escherichia coli

Yamamoto, M.; Nomura, M.

Proceedings of the National Academy of Sciences of the United States of America 75(8): 3891-3895

1978

ISSN/ISBN: 0027-8424
PMID: 358203
DOI: 10.1073/pnas.75.8.3891
Accession: 039678217
The lambdarifd18 transducing phage carries genes for RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) subunits beta and beta' (rpoB,C) and genes for four ribosomal proteins (rplK for L11, rplA for LI, rplJ for L10, and rplL for L7/L12). DNA segments of various sizes, which cover the rifd allele of the rpoB gene, were cloned into lambda vector phages. The hybrid phages were then analyzed for their ability to express the rpoB gene and neighboring ribosomal protein genes in ultraviolet-irradiated lambda-lysogenic and nonlysogenic bacterial hosts. The results show that the rpoB gene is cotranscribed with two neighboring ribosomal protein genes and that in the rpoB,C transcription unit is: promoter, rp1J, rp1L, rpoB, and rpoC.

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Related References

Dennis, P.P. 1977: Transcription patterns of adjacent segments on the chromosome of Escherichia coli containing genes coding for four 50S ribosomal proteins and the beta and beta' subunits of RNA polymerase Journal of Molecular Biology 115(4): 603-625
Dennis, P.P. 1977: Transcription patterns of adjacent segments on the chromosome of escherichia coli containing genes coding for 4 50s ribosomal proteins and the beta and beta prime subunits of rna polymerase Journal of Molecular Biology 115(4): 603-626
Lideman, L.F.; Ponomarenko, O.I.; Shakulov, R.S. 1981: Uncoordinated transcription of genes for beta beta' poly peptides of rna polymerase and adjacent genes for ribosomal proteins in escherichia coli cells Molecular Biology (English Translation of Molekulyarnaya Biologiya (Moscow) 15(2 Part 1): 249-254
Lideman, L.F.; Ponomarenko, O.I.; Shakulov, R.S. 1981: Non-coordinated transcription of RNA polymerase beta,beta'-polypeptide genes and adjacent ribosomal protein genes in Escherichia coli cells Molekuliarnaia Biologiia 15(2): 316-322
Lindahl, L.; Jaskunas, S.R.; Dennis, P.P.; Nomura, M. 1975: Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits Proceedings of the National Academy of Sciences of the United States of America 72(7): 2743-2747
Boyle, S.M.; Chu, F.; Brot, N.; Sells, B.H. 1978: The relationship between the spoT gene, the synthesis of stable RNA, ribosomal proteins, and the beta beta' subunits of RNA polymerase following a nutritional shiftup of Escherichia coli Canadian Journal of Biochemistry 56(6): 528-533
Kirschbaum, J.B.; Scaife, J. 1974: Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase Molecular and General Genetics: Mgg 132(3): 193-201
Fedoseeva, V.B.; Polonskiĭ, I.S.; Aleksandrov, A.A. 1986: Stimulation of the transcription of the RNA polymerase genes of Escherichia coli (beta beta' subunits) in vitro as affected by rifampicin Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 9: 36-43
Fedoseeva, V.B.; Polonskii-Yu, S.; Aleksandrov, A.A. 1986: Transcription stimulation in vitro of escherichia coli genes for beta beta' subunits of rna polymerase in the presence of rifampicin Molekulyarnaya Genetika Mikrobiologiya i Virusologiya 9: 36-43
Kirschbaum, J.B.; Greenblatt, J.; Allet, B.; Rochaix J D. 1976: Studies with a lambda rif transducing phage carrying the genes for the beta and beta prime subunits of escherichia coli rna polymerase Losick, Richard And Michael Chamberlin (Ed ) Rna Polymerase Ix+899p Illus Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y , U S A ISBN 0-87969-115-8: 503-518
Maher, D.L.; Dennis, P.P. 1977: In vivo transcription of escherichia coli genes coding for ribosomal rna ribosomal proteins and subunits of rna polymerase influence of the stringent control system Molecular and General Genetics 155(2): 203-212
Von Gabain, A.; Simon, M.; Matzura, H. 1975: Characterization of the restriction fragments of the dna of a transduced lambda phage with the genes for the beta and beta prime subunits of the rna polymerase from escherichia coli Hoppe-Seyler's Zeitschrift fuer Physiologische Chemie 356(3): 233
Takto, M.; Ishihama, A.; Kirschbaum, J.B. 1976: Altered synthesis and stability of RNA polymerase holoenzyme subunits in mutants of Escherichia coli with mutations in the beta or beta' subunit genes Molecular and General Genetics: Mgg 147(2): 139-143
Taketo, M.; Ishihama, A.; Krischbaum, J.B. 1976: Altered synthesis and stability of rna polymerase ec 2.7.7.6 holo enzyme subunits in mutants of escherichia coli with mutations in the beta or beta prime subunit genes Molecular and General Genetics 147(2): 139-144
Patrick, P. Dennis 1977: Transcription patterns of adjacent segments on the chromosome of Escherichia coli containing genes coding for four 50 S ribosomal proteins and the b and b subunits of RNA polymerase Journal of Molecular Biology 115(4): 603-620
Solov'ev, V.V.; Zharkikh, A.A.; Kolchanov, N.A. 1985: Context analysis of polynucleotide sequences. Methods of detecting non-random repeats. I. Direct repeats in genes of beta-, beta'-, sigma subunits of Escherichia coli RNA-polymerase Molekuliarnaia Biologiia 19(2): 524-536
Aboshkiwa, M.; al-Ani, B.; Coleman, G.; Rowland, G. 1992: Cloning and physical mapping of the Staphylococcus aureus rplL, rpoB and rpoC genes, encoding ribosomal protein L7/L12 and RNA polymerase subunits beta and beta' Journal of General Microbiology 138(9): 1875-1880
Severinov, K.; Mustaev, A.; Kukarin, A.; Muzzin, O.; Bass, I.; Darst, S.A.; Goldfarb, A. 1996: Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase Journal of Biological Chemistry 271(44): 27969-27974
Mekhedov, S.L.; Bass, I.A. 1986: Similar arrangement of the beta beta' subunit rna polymerase genes and adjacent ribosomal protein genes in enterobacteria and pseudomonas putida Molekulyarnaya Biologiya (Moscow) 20(1): 92-101
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