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Chapter 39,686

Convenient vectors for cloning and sequencing EcoRi and HindIIi fragments

Edenberg, H.J.; Moss, L.G.; Rutter, W.J.

Gene 58(2-3): 297-298

1987

ISSN/ISBN: 0378-1119
PMID: 2828191
DOI: 10.1016/0378-1119(87)90384-2
Accession: 039685060
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The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.

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