+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus E1 glycoprotein expressed from cloned cDNA localizes in the Golgi region



Coronavirus E1 glycoprotein expressed from cloned cDNA localizes in the Golgi region



Journal of Virology 61(6): 2042-2045



Cloned cDNA encoding the membrane glycoprotein E1 of the coronavirus mouse hepatitis virus strain A59 was expressed transiently in a monkey fibroblast cell line (COS) by using a simian virus 40-based vector. As determined by indirect immunofluorescence microscopy, the E1 protein accumulated intracellularly in a perinuclear region coincident with a Golgi marker. The same three species of E1 that occur in virus-infected cells were also found in transfected cells. These are one unglycosylated form and two apparently O-glycosylated forms that could be labeled in a tunicamycin-resistant fashion with [3H]glucosamine. Because O glycosylation occurs posttranslationally in the Golgi apparatus, we could show, by monitoring the rate of acquisition of oligosaccharides, that the transport of E1 from the rough endoplasmic reticulum to the Golgi apparatus had a half time of between 15 and 30 min.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039691309

Download citation: RISBibTeXText

PMID: 3033331


Related references

A specific transmembrane domain of a coronavirus E1 glycoprotein is required for its retention in the Golgi region. Journal of Cell Biology 105(3): 1205-1214, 1987

Differentiation of transmissible gastroenteritis virus from porcine respiratory coronavirus and other antigenically related coronaviruses by using cDNA probes specific for the 5' region of the S glycoprotein gene. Journal of Clinical Microbiology 29(1): 215-218, 1991

Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nature Genetics 4(4): 373-380, 1993

Drosophila protein kinase D is broadly expressed and a fraction localizes to the Golgi compartment. Gene Expression Patterns 6(8): 849-856, 2006

A strategy for the generation of an infectious transmissible gastroenteritis coronavirus from cloned cDNA. Advances in Experimental Medicine and Biology 494: 261-266, 2001

The E1 glycoprotein of an avian coronavirus is targeted to the cis Golgi complex. Proceedings of the National Academy of Sciences of the United States of America 87(18): 6944-6948, 1990

Primary sequence requirements for retention of a coronavirus e1 glycoprotein in the golgi complex. Journal of Cell Biology 107(6 Part 3): 772A, 1988

The EF-hand Ca2+binding protein p22 localizes to the Golgi region. Molecular Biology of the Cell 11(Suppl.): 133a-134a, 2000

Radioactive and enzymatic cloned cDNA probes for bovine enteric coronavirus detection by molecular hybridization. Archives of Virology 125(1-4): 25-37, 1992

Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. Journal of General Virology 82(Pt 6): 1273-1281, 2001

Molecular analysis of Brazilian strains of bovine coronavirus (BCoV) reveals a deletion within the hypervariable region of the S1 subunit of the spike glycoprotein also found in human coronavirus OC43. Archives of Virology 151(9): 1735-1748, 2006

Localization to the Golgi complex of Uukuniemi virus glycoproteins G1 and G2 expressed from cloned cDNAs. Journal of Virology 66(7): 4525-4531, 1992

Caveolin-2 localizes to the golgi complex but redistributes to plasma membrane, caveolae, and rafts when co-expressed with caveolin-1. Journal of Biological Chemistry 274(36): 25708-25717, 1999

Immunogenicity of a recombinant coronavirus spike glycoprotein expressed in transgenic plants. Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz 43(2): 110-115, 2000

Characterization of human prorenin expressed in mammalian cells from cloned cDNA. Proceedings of the National Academy of Sciences of the United States of America 83(12): 4114-4118, 1986