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Cresyl violet: a red fluorescent Nissl stain


Cresyl violet: a red fluorescent Nissl stain



Journal of Neuroscience Methods 33(2-3): 129-133



ISSN/ISBN: 0165-0270

PMID: 2232864

DOI: 10.1016/0165-0270(90)90016-9

Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy.

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Accession: 039705811

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