EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Cyclic GMP mediates the agonist-stimulated increase in plasma membrane calcium entry in the pancreatic acinar cell


Cyclic GMP mediates the agonist-stimulated increase in plasma membrane calcium entry in the pancreatic acinar cell



Journal of Biological Chemistry 265(22): 12846-12853



ISSN/ISBN: 0021-9258

PMID: 2165487

The present studies were performed to determine the role of cyclic GMP in regulating agonist mediated calcium entry in the pancreatic acinar cell. In guinea pig-dispersed pancreatic acini the findings demonstrated that carbachol stimulated a transient 20-40-fold rise in cellular cyclic GMP followed by a sustained 3-4-fold rise in cellular cyclic GMP. The guanylate cyclase inhibitor, 6-anilino-5,8-quinolinedione (LY83583), caused a dose-dependent inhibition of carbachol-stimulated increases in cellular cyclic GMP both during the initial transient large increase in cyclic GMP and the sustained increase in cyclic GMP. LY83583 also inhibited cellular Ca2+ influx during carbachol stimulation and reloading of the agonist-sensitive pool of Ca2+ at the termination of carbachol stimulation with atropine. The effect of the inhibition on reloading of the agonist-sensitive pool was secondary to its effects on the plasma membrane C2+ entry. The addition of dibutyryl cyclic GMP to LY83583-treated acini restored Ca2+ influx across the plasma membrane. Nitroprusside increased both cellular cyclic GMP and the rate of Ca2+ influx. During periods when plasma membrane Ca2+ entry was activated, cellular cyclic GMP levels were increased. These results suggest that agonist-induced increases in cellular cyclic GMP are necessary and sufficient to mediate the effects of the agonist on the plasma membrane Ca2+ entry mechanism.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039724394

Download citation: RISBibTeXText

Related references

The agonist sensitivity calcium pool in the pancreatic acinar cell activation of plasma membrane calcium influx mechanism. Journal of Biological Chemistry 262(35): 16963-16968, 1987

The agonist-sensitive calcium pool in the pancreatic acinar cell. Activation of plasma membrane Ca2+ influx mechanism. Journal of Biological Chemistry 262(35): 16963-16968, 1987

Cellular mechanisms mediating agonist-stimulated calcium influx in the pancreatic acinar cell. Annals of the new York Academy of Sciences 713: 41-48, 1994

Th agonist sensitive pool of calcium regulates cellular cyclic gmp in the pancreatic acinar cell. Gastroenterology 102(4 Part 2): A284, 1992

Calcium-stimulated ATPase activity in plasma membrane vesicles from pancreatic acinar cells. Biochemical and Biophysical Research Communications 156(2): 746-751, 1988

Cyclic gmp regulates plasma membrane calcium pump activity in pancreatic acinar cells. Gastroenterology 96(5 Part 2): A382, 1989

Wortmannin a specific inhibitor of myosin light chain kinase prevents the agonist stimulated plasma membrane calcium ion entry in human platelets. Japanese Journal of Pharmacology 55(Suppl 1): 91P, 1991

Agonist-stimulated pathways of calcium signaling in pancreatic acinar cells. Biological Chemistry 380(7-8): 903-908, 1999

The control of plasma membrane calcium channel activation in the pancreatic acinar cell. Gastroenterology 92(5 Part 2): 1565, 1987

Cyclic GMP stimulates depletion-activated calcium entry in rat pancreatic acinar cells. Biophysical Journal 64(2 Part 2): A127, 1993

Inositol trisphosphate-independent agonist-stimulated calcium influx in rat pancreatic acinar cells. Journal of Biological Chemistry 268(27): 20237-20242, 1993

Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane. Journal of Biological Chemistry 264(21): 12266-12271, 1989

S1692 Cyclic-Amp Accelerates Carbachol- and Caerulein-Stimulated Calcium Waves in Pancreatic Acinar Cells. Gastroenterology 134(4): A-251, 2008

Quantal calcium release and calcium entry in the pancreatic acinar cell. Yale Journal of Biology and Medicine 65(5): 399-405; discussion 437-40, 1992

Extracellular Histones Activate Plasma Membrane Toll-Like Receptor 9 to Trigger Calcium Oscillations in Rat Pancreatic Acinar Tumor Cell AR4-2J. Cells 8(1), 2018