Section 40
Chapter 39,730

Cytochrome b556 complexes solubilized from Micrococcus lysodeikticus membranes by triton X-100

Turgenbaeva, D.A.; Kharat'ian, E.F.; Zhukova, I.G.; Lukoianova, M.A.; Ostrovskii, D.N.

Biokhimiia 44(4): 729-737


ISSN/ISBN: 0320-9725
PMID: 435582
Accession: 039729474

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The integral protein of cytochrome b556 after its solubilization with Triton X-100 from M. lysodeikticus membranes was studied. The cytochrome was found in complexes differing in charge and size during preparative gel electrophoresis and centrifugation in a sucrose concentration gradient. Cytochrome b556, being in complexes, retains its ability to be reduced by NADH dehydrogenase. The electron micrographs of the membranes after solubilization by Triton X-100 demonstrated the maintenance of the membrane structure. It is concluded that native protein complexes marked with cytochrome b556 are extracted from the membranes under their solubilization.

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