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Cytochrome o (bo) is a proton pump in Paracoccus denitrificans and Escherichia coli



Cytochrome o (bo) is a proton pump in Paracoccus denitrificans and Escherichia coli



Febs Letters 249(2): 163-167



Spheroplasts from aerobically grown wild-type Paracoccus denitrificans cells respire with succinate despite specific inhibition of the cytochrome bc1 complex by myxothiazol. Coupled to this activity, which involves only b-type cytochromes, there is translocation of 1.5-1.9 h+/e- across the cytoplasmic membrane. Similar H+ translocation ratios are observed during oxidation of ubiquinol in spheroplasts from aerobically grown mutants of Paracoccus lacking cytochrome c oxidase, or deficient in cytochrome c, as well as in a strain of E. coli from which cytochrome d was deleted. These observations show that the cytochrome o complex is a proton pump much like cytochrome aa3 to which it is structurally related.

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Accession: 039729579

Download citation: RISBibTeXText

PMID: 2544445

DOI: 10.1016/0014-5793(89)80616-7



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