+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Cytochrome o (bo) is a proton pump in Paracoccus denitrificans and Escherichia coli

Cytochrome o (bo) is a proton pump in Paracoccus denitrificans and Escherichia coli

Febs Letters 249(2): 163-167

Spheroplasts from aerobically grown wild-type Paracoccus denitrificans cells respire with succinate despite specific inhibition of the cytochrome bc1 complex by myxothiazol. Coupled to this activity, which involves only b-type cytochromes, there is translocation of 1.5-1.9 h+/e- across the cytoplasmic membrane. Similar H+ translocation ratios are observed during oxidation of ubiquinol in spheroplasts from aerobically grown mutants of Paracoccus lacking cytochrome c oxidase, or deficient in cytochrome c, as well as in a strain of E. coli from which cytochrome d was deleted. These observations show that the cytochrome o complex is a proton pump much like cytochrome aa3 to which it is structurally related.

(PDF emailed within 0-6 h: $19.90)

Accession: 039729579

Download citation: RISBibTeXText

PMID: 2544445

DOI: 10.1016/0014-5793(89)80616-7

Related references

Proton pump coupled to cytochrome c oxidase in Paracoccus denitrificans. Biochimica et Biophysica Acta 635(3): 525-534, 1981

An alternative cytochrome oxidase of Paracoccus denitrificans functions as a proton pump. Biochimica Et Biophysica Acta. 1186(1-2): 100-106, 1994

An elementary reaction step of the proton pump is revealed by mutation of tryptophan-164 to phenylalanine in cytochrome c oxidase from Paracoccus denitrificans. Biochemistry 44(50): 16502-16512, 2005

Alteration of haem-attachment and signal-cleavage sites for Paracoccus denitrificans cytochrome c-550 probes pathway of c-type cytochrome biogenesis in Escherichia coli. Molecular Microbiology. : 1193-1204, 1996

Subunit ii of the paracoccus denitrificans cytochrome c oxidase expression studies of its cloned gene in escherichia coli. Brunori, M And B Chance (Ed ) Annals Of The New York Academy Of Sciences, Vol 550 Cytochrome Oxidase: Structure, Function, And Physiopathology; Conference, Rome, Italy, June 20-21, X+382p The New York Academy Of Sciences: New York, New York, Usa Illus 308-313, 1988

Structural and functional analysis of aa-3-type and cbb-3-type cytochrome c oxidases of Paracoccus denitrificans reveals significant differences in proton-pump design. Molecular Microbiology 20(6): 1247-1260, 1996

Expression of plastocyanin and cytochrome f of the cyanobacterium Phormidium laminosum in Escherichia coli and Paracoccus denitrificans and the role of leader peptides. Gene (Amsterdam) 234(2): 275-283, July 8, 1999

Proton nmr spectroscopy of paracoccus denitrificans cytochrome c 550. Biochemistry 23(15): 3526-3533, 1984

Dicyclohexylcarbodiimide does not inhibit proton pumping by cytochrome c oxidase of Paracoccus denitrificans. European Journal of Biochemistry 134(1): 33-37, 1983

The role of the conserved tryptophan272 of the Paracoccus denitrificans cytochrome c oxidase in proton pumping. Biochimica et Biophysica Acta 1777(7-8): 925-928, 2008

Electron and proton transfer in the arginine-54-methionine mutant of cytochrome c oxidase from Paracoccus denitrificans. Biochemistry (American Chemical Society) 40(17): 69-74, 2001