+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

DNA damage induced by nitropyrenes in primary mouse hepatocytes and in rat H4-II-E hepatoma cells

DNA damage induced by nitropyrenes in primary mouse hepatocytes and in rat H4-II-E hepatoma cells

Mutation Research 151(1): 137-146

The capacity of nitropyrenes to cause DNA damage in primary mouse hepatocytes (C57BL/6N mice) and rat H4-II-E hepatoma cells was studied by estimating single-strand breaks using the alkaline elution technique. 1-Nitropyrene (10-200 microM) caused clear dose-dependent increases in DNA strand breaks in both cell types, whereas no increase in DNA strand breaks was observed in hepatocytes treated with 1.3-, 1,6-, 1,8-dinitropyrene, 1,3,6-trinitropyrene and 1,3,6,8-tetranitropyrene under standard assay conditions (5-20 microM 30-min incubation). However, 1,8-dinitropyrene (1,8-DNP) caused dose-dependent increases in DNA strand breaks when incubated with the H4-II-E cells for 48 h, while no single-strand breaks were observed following treatment with 1,6-dinitropyrene (1,6-DNP) under the same conditions. Neither 1,6-DNP nor 1,8-DNAP induced DNA crosslinks in the H4-II-E cells. These data indicate that substrate specificity exists in the metabolic activation of nitropyrenes in murine liver.

(PDF emailed within 0-6 h: $19.90)

Accession: 039737367

Download citation: RISBibTeXText

PMID: 4022037

DOI: 10.1016/0027-5107(85)90192-7

Related references

Cytotoxic effects of propiconazole and its metabolites in mouse and human hepatoma cells and primary mouse hepatocytes. Toxicology in Vitro 22(6): 1476-1483, 2008

Turnover of coated vesicle associated glyco proteins in rat hepatoma cells and primary culture of mouse hepatocytes. Journal of Cell Biology 91(2 PART 2): 114A, 1981

The retinoblastoma gene product inhibits TGF-beta1 induced apoptosis in primary rat hepatocytes and human HuH-7 hepatoma cells. Oncogene 12(9): 1909-1919, 1996

Use of primary hepatocytes and hepatoma cells to assess drug-induced intracellular oxidative stress by flow cytometry. Cytometry Supplement (10): 105, 2000

Protective effect of Cistanchis A on ethanol-induced damage in primary cultured mouse hepatocytes. Biomedicine & PharmacoTherapy 83(): 1071-1079, 2016

Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis. Environmental Science and Pollution Research International 23(2): 1335-1343, 2016

Toxicity of erythromycin, paracetamol and isoniazid in human hepatocytes in primary culture and in the human hepatoma cell line HepG-2 Comparison with rat and mouse hepatocytes in primary culture. Cell Biology & Toxicology 11(3-4): 195, 1995

Phosphatidylinositol-3-kinase p110γ contributes to bile salt-induced apoptosis in primary rat hepatocytes and human hepatoma cells. Journal of Hepatology 53(5): 918-926, 2011

Characterisation of cisplatin-induced transcriptomics responses in primary mouse hepatocytes, HepG2 cells and mouse embryonic stem cells shows conservation of regulating transcription factor networks. Mutagenesis 29(1): 17-26, 2014

Effects of the retinoblastoma gene product on transforming growth factor-beta-1-induced apoptosis in primary rat hepatocytes and human hepatoma HuH-7 cells. Proceedings of the American Association for Cancer Research Annual Meeting 37(0): 15, 1996

Hormonal regulation of carbamoyl-phosphate synthetase I synthesis in primary cultured hepatocytes and Reuber hepatoma H-35. Defective regulation in hepatoma cells. European Journal of Biochemistry 167(1): 19-25, 1987

Synthesis of vaccinia specified antigens in mouse hepatocytes after frog virus 3 induced damage to the sinusoidal cells. Fems Microbiology Letters: 5-7, 1978

Effects of borneol on the level of DNA damage induced in primary rat hepatocytes and testicular cells by hydrogen peroxide. Food and Chemical Toxicology 47(6): 1318-1323, 2009

Mouse interferon gamma pretreated hepatocytes conditioned media suppress cytochrome P-450 induction by TCDD in mouse hepatoma cells. Biochemistry and Molecular Biology International 29(2): 197-202, 1993

The influence of glutathione and detoxifying enzymes on DNA damage induced by 2-alkenals in primary rat hepatocytes and human lymphoblastoid cells. Chemical Research in Toxicology 8(1): 40-46, 1995