EurekaMag
+ Translate
+ Most Popular
Spermatogenic and steroidogenic impairment after chromium treatment in rats
Prediction of enthalpy of formation based on refined crystal structures of multisite compounds; Part 1, Theories and examples
Intrastromal photorefractive keratectomy for myopia by Nd:YLF picosecond laser
Teachers' beliefs about the role of prior language knowledge in learning and how these influence teaching practices
Classification and genesis of submarine iron-manganese deposits
Insecta a Cl. G. N. Potanin in China et in Mongolia novissime lecta. XVII. Hemiptera-Heteroptera
On the occurrence of the splendid genus Machaerothrix Haupt in Tsushima, with some remarks on the genus (Hymenoptera, Pompilidae)
Enamel craze lines
Gynecologic examination of the child and the adolescent
Periosteal osteoclasts, skeletal development and ageing
Fibrodysplasia ossificans progressiva in a Nigerian: a case report
The chronically ill patient with anorexia nervosa development, phenomenology, and therapeutic considerations
Kummelonautilus taiwanum sp. nov. (Nautilida) from the early Miocene Shihmen Formation, Chungliao, Nantou, west-central Taiwan
Efficacy of Droncit against Dipylidium caninum in dogs
Pierids of the genus Euchloe Klots (Lepidoptera, Pieridae) from Siberia and the Far East.
C5A anaphylatoxin and its seven transmembrane-segment receptor
Failure to practice evidence-based medicine: why do physicians not treat patients with heart failure with angiotensin-converting enzyme inhibitors?
Empirical comparisons of proportional hazards regression, Poisson regression, and logistic regression in the analysis of occupational cohort data
Pollination of flowers of sweet and sour cherries
Isopodi terrestri raccolti nell' Estremo Oriente
Morphometric observations on Dipylidium caninum from dogs and cats in Austria
Chelation effectiveness of zinc proteinates demonstrated
Alopecia--a psychosomatic disease picture? I. Review of the literature
Monitoring equity in health and healthcare: a conceptual framework
Hemodynamic evaluation of the Biocor heterologous prosthesis

Detection of benzo[a]pyrene-DNA adducts in cultured cells treated with benzo[a]pyrene diol-epoxide by quantitative immunofluorescence microscopy and 32P-postlabelling; immunofluorescence analysis of benzo[a]pyrene-DNA adducts in bronchial cells from smoking individuals


Detection of benzo[a]pyrene-DNA adducts in cultured cells treated with benzo[a]pyrene diol-epoxide by quantitative immunofluorescence microscopy and 32P-postlabelling; immunofluorescence analysis of benzo[a]pyrene-DNA adducts in bronchial cells from smoking individuals



Iarc Scientific Publications 89: 146-154



ISSN/ISBN: 0300-5038

PMID: 3143666

Monoclonal antibodies were raised against the reaction product of benzo[a]pyrene diol-epoxide (BPDE) and deoxyguanosine-5'-monophosphate. The antibodies were used for detection of DNA adducts in situ in BPDE-treated cultured human fibroblasts by immunofluorescence microscopy. Analogue-digital conversion of the fluorescence signal and further image processing allowed measurement of the immunospecific fluorescence in the nuclei of these cells. The results are compared with the adduct levels measured in isolated DNA by 32P-postlabelling. Preliminary results are shown of the application of the immunofluorescence method to the analysis of DNA adducts in bronchial cells obtained from smoking individuals.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039774166

Download citation: RISBibTeXText

Related references

Differential c-myc expression profiles in normal human bronchial epithelial cells following treatment with benzo[a]pyrene, benzo[a]pyrene-4,5 epoxide, and benzo[a]pyrene-7,8-9,10 diol epoxide. Molecular Carcinogenesis 40(2): 79-89, 2004

Quantitative determination of benzo a pyrene dna adducts in tissues of mice with polyclonal antibodies recognizing dna modified by benzo a pyrene diol epoxide. Proceedings of the American Association for Cancer Research Annual Meeting 28: 93, 1987

Detection of antibodies to benzo a pyrene diol epoxide dna adducts following occupational exposure to benzo a pyrene. Journal of Cellular Biochemistry Suppl (9 Part C): 47, 1985

Synchronous fluorescence spectrophotometry of benzo[a]pyrene diol epoxide-DNA adducts: a tool for detection of in-vitro and in-vivo DNA damage by exposure to benzo[a]pyrene. Iarc Scientific Publications 89: 208-212, 1988

A comparative investigation of DNA adducts, DNA strand breaks and gene mutations induced by benzo[a]pyrene and (+/-)-anti-benzo[a]pyrene-7,8-diol 9,10-oxide in cultured human cells. Mutation Research 390(1-2): 179-188, 1997

Exposure to benzo[a]pyrene of Hepatic Cytochrome P450 Reductase Null (HRN) and P450 Reductase Conditional Null (RCN) mice: Detection of benzo[a]pyrene diol epoxide-DNA adducts by immunohistochemistry and 32P-postlabelling. Toxicology Letters 213(2): 160-166, 2012

Analysis of the repair of benzo pyrene and dibenzo pyrene-diol epoxide-DNA adducts in human and rodent cells. Proceedings of the American Association for Cancer Research Annual Meeting 39: 637, 1998

Reaction of benzo a pyrene and benzo a pyrene diol epoxide with hemoglobin and the chromatographic resolution of the covalent adducts. Proceedings of the American Association for Cancer Research Annual Meeting 33: 102, 1992

Genetic polymorphisms in biotransformation enzymes for benzo[a]pyrene and related levels of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts in Goeckerman therapy. Toxicology Letters 255: 47-51, 2016

Topical treatment of mice with benzo[a]pyrene or parenteral administration of benzo[a]pyrene diol epoxide-DNA to rats results in faecal excretion of putative benzo[a]pyrene diol epoxide-deoxyguanosine adduct. Carcinogenesis (New York. Print) 8(9): 1189-1192, 1987

Topical treatment of mice with benzo[a]pyrene or parenteral administration of benzo[a]pyrene diol epoxide-DNA to rats results in faecal excretion of a putative benzo[a]pyrene diol epoxide-deoxyguanosine adduct. Carcinogenesis 8(9): 1189-1192, 1987

The binding efficiency of polyclonal and monoclonal antibodies to DNA modified with benzo[a]pyrene diol epoxide is dependent on the level of modification. Implications for quantitation of benzo[a]pyrene-DNA adducts in vivo. Carcinogenesis 8(9): 1263-1269, 1987

The binding efficiency of polyclonal and monoclonal antibodies to DNA modified with benzo[a]pyrene diol epoxide is dependent on the level of modification. Implications for quantitation on benzo[a]pyrene-DNA adducts in vivo. Carcinogenesis (New York. Print) 8(9): 1263-1269, 1987

Quantitative analysis of 3-hydroxybenzo[a] pyrene and (+)-anti-benzo[a] pyrene-diolepoxide-DNA adducts in benzo[a] pyrene induced mice by the protection of lemongrass essential oil. Analytical Methods 9(27): 4091-4100, 2017

Differences in the dna adducts formed in cultured rabbit and rat dermal fibroblasts by benzo a pyrene and levo benzo a pyrene 7 8 diol. Cancer Research 48(24 Part 1): 7132-7139, 1988