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Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridisation



Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridisation



Journal of Medical Virology 29(1): 70-73



A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-stranded RNA probe. Nucleic acid hybridisation is shown to be a sensitive technique for the diagnosis of HCV 229E infections.

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Accession: 039775710

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PMID: 2584959


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