+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of human glioma-associated antigen by rat monoclonal antibody raised against syngeneic rat glioma cells



Detection of human glioma-associated antigen by rat monoclonal antibody raised against syngeneic rat glioma cells



Journal of Neurosurgery 65(4): 495-502



A monoclonal antibody termed "FR77" was obtained from a hybridoma clone established by fusion between P3x63Ag8.653 mouse myeloma cells and spleen cells of a Fischer F344 rat hyperimmune to syngeneic 9L/R3 glioma cells. Immunoperoxidase staining of various cultured cells showed that FR77 was reactive to both rat and human glioma cells, but was not reactive with other nonglioma cells. Immunohistochemical examination of paraffin-embedded or cryostat-frozen sections of various human tissues revealed that FR77 was strongly reactive with glioblastoma, grade III astrocytoma, and craniopharyngioma; partially reactive with intracerebral primitive neuroectodermal tumor, pineoblastoma, and desmoplastic medulloblastoma; and weakly reactive with low-grade astrocytoma. It was not reactive with other types of brain tumors and normal human tissues tested. The FR77-defined antigen was observed to be predominantly localized in the cytoplasm of antigen-bearing cells as suggested by the immunostaining pattern, but part of it was also expressed on the cell surface of glioma cells as demonstrated by a complement-mediated cytotoxic test. Fractionation of the antigenic component and periodic acid treatment of tumor tissue bearing the FR77-defined antigen indicated that the antigen is of a neutral glycolipid nature and that the antigenic determinant to FR77 is present on its sugar portion.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039775725

Download citation: RISBibTeXText

PMID: 3760959

DOI: 10.3171/jns.1986.65.4.0495


Related references

Analysis of interspecies cross reactive glioma associated antigen by syngeneic monoclonal anti rat glioma antibody. Medical Journal of Kobe University 47(4): 281-288, 1986

Radioimaging of human glioma xenografts with 123I labeled monoclonal antibody G-22 against glioma-associated antigen. Journal of Neuro-Oncology 8(3): 221-229, 1990

Anti-(glioma surface antigen) monoclonal antibody G-22 recognizes overexpressed CD44 in glioma cells. Cancer Immunology Immunotherapy 39(5): 313-317, 1994

Radioimaging of human glioma by indium-111 labeled G-22 anti-glioma monoclonal antibody. Noshuyo Byori 12(2): 105-110, 1995

Derivation of a brain tumor-selective monoclonal antibody from hybridoma between mouse myeloma and rat spleen cells immune to syngeneic glioma. Japanese Journal of Cancer Research 76(12): 1198-1202, 1985

Immunopathological investigation of human glioma-associated antigen (GAA) detected by monoclonal antibody. Zhonghua Yi Xue Za Zhi 41(2): 89-96, 1988

Characterization of neuroectodermal antigen by a monoclonal antibody and its application in CSF diagnosis of human glioma. Journal of Neurosurgery 68(3): 449-455, 1988

Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody. Cancer Research 43(6): 2796-2805, 1983

Recognition of an apparently ubiquitous glioma surface antigen by human monoclonal antibody BT32/A6. Funatsu, K, Shirai, Y, Matsushita, T Animal Cell Technology: 471-477, 1997

Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen. Journal of Neurosurgery 82(3): 475-480, 1995

Partial purification and characterization of a murine glioma-associated antigen defined by syngeneic rat monoclonal antibodies. Journal of Neuroimmunology 13(2): 203-216, 1986

Differential glioma-associated tumor antigen expression profiles of human glioma cells grown in hypoxia. Plos one 7(9): E42661, 2012

The studies on monoclonal antibody SZ-39 against human glioma cells. Chinese Journal of Cancer Research 1(4): 5-10, 1989

Internalization with high targeting potential of mouse monoclonal antibody ONS-M21 recognizing human malignant glioma antigen. Cancer Letters. 127(1-2): 171-176, 15, 1998

Liposomes coupled with monoclonal antibodies against glioma-associated antigen for targeting chemotherapy of glioma. Journal of Neurosurgery 71(3): 382-387, 1989