Section 40
Chapter 39,780

Determination of L-alanosine in plasma and urine by reversed-phase high-performance liquid chromatography of the Dns derivative

Powis, G.; Ames, M.M.

Journal of Chromatography 170(1): 195-201


ISSN/ISBN: 0021-9673
PMID: 544618
DOI: 10.1016/s0021-9673(00)84251-x
Accession: 039779908

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L-Alanosine is an antitumour antibiotic that has recently been placed in clinical trial. We have developed a relatively rapid and specific assay for urinary and plasma alanosine, based on formation of the Dns derivative and separation of this from other Dns compounds by reversed-phase high-performance liquid chromatography. Dns-Alanosine is detected by its absorption at 254 nm, since alanosine is atypical in that it forms a Dns derivative with very low fluorescence. The lower limit of detection of alanosine in plasma is 0.1 microgram/ml. The assay has been used to measure the levels of alanosine in the plasma and urine of rabbits and of man.

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