Determination of delta 9-tetrahydrocannabinol in plasma using solid-phase extraction and high-performance liquid chromatography with electrochemical detection

Zweipfenning, P.G.; Lisman, J.A.; van Haren, A.Y.; Dijkstra, G.R.; Holthuis, J.J.

Journal of Chromatography 456(1): 83-91

1988


ISSN/ISBN: 0021-9673
PMID: 2854139
DOI: 10.1016/0021-9673(86)80008-5
Accession: 039781849

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Abstract
A method for the determination of nanogram amounts of delta 9-tetrahydrocannabinol (THC) in plasma and serum is described. THC was quantitatively isolated by solid-phase extraction after addition of an aqueous solution of urea and methanol to the sample. The extracts were analysed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. The detection limit of THC is ca. 100 pg for a signal-to-noise ratio of 3. With this method, levels of 2 ng/ml of THC in plasma can be measured.