Determination of leukocyte elastase-inhibitor complexes and leukocyte neutral proteinase inhibitor by enzyme immunoassays. Leukocyte elastase-inhibitor complexes in porcine blood, IIi

Geiger, R.; Sokal, S.; Siebeck, M.; Hoffmann, H.; Trefz, G.

Journal of Clinical Chemistry and Clinical Biochemistry 26(10): 605-609

1988


ISSN/ISBN: 0340-076X
PMID: 3230378
DOI: 10.1515/cclm.1988.26.10.605
Accession: 039783412

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Abstract
Sensitive enzyme immunoassays for the determination of total leukocyte neutral proteinase inhibitor and polymorphonuclear elastase-leukocyte neutral proteinase inhibitor complexes are described. The usable ranges of the standard curves were from 80 ng/l to 10 micrograms/l. The relative intra-assay coefficients of variation of the tests were between 2 and 4%, and the inter-assay coefficients of variation between 4 and 10%. In vitro and in vivo studies were performed with septic pigs and isolated leukocytes. The results show that leukocyte neutral proteinase inhibitor can be used in pigs as a parameter for the development of a septicaemia.