Differential assay of salivary and pancreatic alpha-amylase in serum and urine, with use of monoclonal antibody to human salivary amylase immobilized on bacterial cell wall

Hiroishi, S.; Matsuyama, S.; Kurooka, S.; Sunahara, N.; Inoue, S.; Ida, T.

Clinical Chemistry 33(7): 1235-1236

1987


ISSN/ISBN: 0009-9147
PMID: 3496178
Accession: 039822547

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Abstract
We previously reported (Clin Chim Acta 1986;159:89) that bacterial cell wall chemically coated with a monoclonal antibody specific to human salivary (S) amylase (EC 3.2.1.1) could be successfully used to separate S and pancreatic (P) amylase in solution. We have now applied this method to serum and urine samples and found that the activities of S and P amylases so measured correlated well with those measured by the isoamylase inhibitor method. The present method is simple and reliable for routine clinical tests.