+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Diphtheria toxin promoter function in Corynebacterium diphtheriae and Escherichia coli



Diphtheria toxin promoter function in Corynebacterium diphtheriae and Escherichia coli



Nucleic Acids Research 13(9): 3147-3159



The expression of the diphtheria tox228 gene encoding the nontoxic, serologically related CRM228 mutant diphtheria toxin has been analyzed in Corynebacterium diphtheriae and Escherichia coli. The diphtheria toxin promoter has been used to direct the expression of beta-galactosidase in E.coli, and the efficiency of promotion has been compared to that obtained with the lac promoter. Expression in C.diphtheriae is known to be dependent on the absence of iron, and we present for the first time direct evidence that this regulation occurs at the level of transcription. The 5' end of toxin mRNA maps at the same position in C.diphtheriae and E.coli, suggesting identical sequences to be recognized by C.diphtheriae and E.coli RNA polymerase. The diphtheria toxin promoter carries at position -34 a TTGATT sequence closely related to the E.coli -35 consensus sequence and in the -14 to -8 region a set of overlapping sequences with complete or partial homology to the E.coli -10 consensus sequence.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039835752

Download citation: RISBibTeXText

PMID: 3923442

DOI: 10.1093/nar/13.9.3147


Related references

Transcription of a mutant diphtheria toxin gene crm 228 in corynebacterium diphtheriae and escherichia coli. Journal of Cellular Biochemistry Suppl. (9 Part B): 180, 1985

Cloning the structural gene for diphtheria toxin from corynebacterium diphtheriae pw8 and expression of its deleted derivative in escherichia coli. Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (11): 13-18, 1987

Iron dependent regulation of diphtheria toxin and siderophore expression by the cloned corynebacterium diphtheriae repressor gene dtxr in strains of corynebacterium diphtheriae c7. Abstracts of the General Meeting of the American Society for Microbiology 91: 27, 1991

Iron dependent regulation of diphtheria toxin and siderophore expression by the cloned corynebacterium diphtheriae repressor gene dtxr in corynebacterium diphtheriae c7 strains. Infection & Immunity 59(6): 1899-1904, 1991

Point mutations in tox promoter/operator and diphtheria toxin repressor (DTXR) gene associated with the level of toxin production by Corynebacterium diphtheriae strains isolated in Belarus. Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 2007(1): 22-29, 2007

Point mutations in tox promoter/operator and diphtheria toxin repressor (Delta txr) gene associated with the level of toxin production by Corynebacterium diphtheriae strains isolated in Belarus. Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (1): 22-29, 2007

Analysis of the two promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor and iron. Abstracts of the General Meeting of the American Society for Microbiology 94: 127, 1994

Characterization of the diphtheria tox transcript in Corynebacterium diphtheriae and Escherichia coli. Journal of Bacteriology 163(3): 1114-1119, 1985

Identification and characterization of three new promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron. Infection and Immunity 65(10): 4273-4280, 1997

Regulation of diphtheria tox operon by iron in corynebacterium diphtheriae and escherichia coli. Abstracts of the Annual Meeting of the American Society for Microbiology 88: 38, 1988

Transformation of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and Escherichia coli with the C. diphtheriae plasmid pNG2. Proceedings of the National Academy of Sciences of the United States of America 84(14): 4964-4968, 1987

Cloning of the structural gene for diphtheria toxin from Corynebacterium diphtheriae PW8 and expression of its deletion derivative in E. coli. Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1987(11): 13-18, 1987

Cloning, sequence, and footprint analysis of two promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron. Journal of Bacteriology 176(4): 1141-1149, 1994

Diphtheria corynebacterium diphtheriae toxin binding glyco proteins on hamster cells candidates for diphtheria toxin receptors. Infection & Immunity 25(3): 786-791, 1979

Diphtheria toxin; the iron enzymes of Corynebacterium diphtheriae and their possible relation to diphtheria toxin. Journal of Biological Chemistry 171(2): 701-713, 1947