+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Direct induction of interferon-gamma- and interferon-alpha/beta-inducible genes by double-stranded RNA



Direct induction of interferon-gamma- and interferon-alpha/beta-inducible genes by double-stranded RNA



Journal of Interferon Research 11(3): 131-141



Using Northern analysis, we here show that the inducibility by double-stranded (ds) RNA of interferon-alpha/beta-inducible genes is not restricted to a few genes but extends to all the genes known to be stimulated by IFN type I in fibroblasts. Moreover, we show that some genes, preferentially regulated by IFN-gamma, are also activated by dsRNA. We present a series of arguments demonstrating that the induction by dsRNA is not mediated by IFN. In addition to the fact that this induction occurs in the presence of cycloheximide and/or anti-IFN-alpha/beta antibodies in fibroblasts, we observed that, in IFN-resistant Daudi cells, ISG15 and IP-10 genes which are not induced by IFN-beta, are still inducible by dsRNA. dsRNA is also still active on 2-5 AS and ISG15 genes in cells carrying homozygous deletions of IFN alpha/beta genes. Actinomycin D experiments and nuclear in vitro elongation assays reveal that the induction by dsRNA involves, as its early step, a transcriptional event. This induction was found not to require protein synthesis, suggesting that activation of preexisting cellular factors is involved. The opposite inducibility by dsRNA of IFN-beta and 2',5'-oligoadenylate (2-5A) synthetase genes in serum-deprived fibroblasts indicates that pathways of induction by dsRNA of these two genes are not identical. Inhibition by 2-aminopurine of the induction of IFN-inducible mRNAs by IFN-beta or dsRNA suggests the participation of a protein kinase in their mechanism of action.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039837580

Download citation: RISBibTeXText

PMID: 1919073


Related references

Direct induction of interferon gamma inducible and interferon alpha beta inducible genes by double stranded rna. Journal of Interferon Research 11(3): 131-142, 1991

Overlapping patterns of polypeptide induction by interleukin 1 alpha il 1 alpha interferon 1 beta il 1 beta interferon alpha ifn alpha interferon gamma ifn gamma and tumor necrosis factor tnf in human fibroblasts. Proceedings of the American Association for Cancer Research Annual Meeting 28: 341, 1987

Synergism between interferon alpha and interferon gamma in induction of an interferon inducible messenger rna. Journal of Cellular Biochemistry Suppl. (10 Part C): 240, 1986

Isolation of cDNA clones for the interferon-induced 67,000-dalton protein: direct induction of a family of mRNAs by human interferon-alpha and interferon-gamma. Journal of Interferon Research 10(3): 309-319, 1990

Isolation of complementary dna clones for the interferon induced 67000 dalton protein direct induction of a family of messenger rna by human interferon alpha and interferon gamma. Journal of Interferon Research 10(3): 309-320, 1990

Mechanism of interferon action: double-stranded RNA-specific adenosine deaminase from human cells is inducible by alpha and gamma interferons. Virology 210(2): 508-511, 1995

The synergistic effect of human recombinant interferon-alpha 2a in combination with interferon-gamma and the induction of interferon-alpha 2a receptor by interferon-gamma. Journal of Pharmacobio-Dynamics 12(5): 299-304, 1989

Analysis of interferon-inducible genes in PBMC and an interferon-inducible protein in serum of chronically HCV-infected patients responsive or resistant to an interferon-alpha treatment. Journal of Hepatology 30(Suppl. 1): 69, 1999

The augmentation of human natural killer cell activity by interferon-gamma is not associated with the induction of the interferon-alpha-inducible proteins. Journal of Immunology 137(1): 167-171, 1986

Interferon double stranded rna mediated growth inhibition in human fibro sarcoma and carcinoma cell lines induction of resistant variants which retain sensitivity to interferon double stranded rna toxicity. Proceedings of the American Association for Cancer Research & American Society of Clinical Oncology 22: 236, 1981

Induction of type I interferon genes and interferon-inducible genes in embryonal stem cells devoid of interferon regulatory factor 1. Proceedings of the National Academy of Sciences of the United States of America 90(24): 11503-7, 1993

Neutralization of interferon-alpha/beta-inducible genes and downstream effect in a phase I trial of an anti-interferon-alpha monoclonal antibody in systemic lupus erythematosus. Arthritis and Rheumatism 60(6): 1785-1796, 2009

Alpha interferon induction of remissions in cml may be mediated by altered binding of nuclear proteins to 5 regulatory regions of interferon inducible genes. Clinical Research 37(2): 601A, 1989

Tumor necrosis factor-alpha and interferon-gamma, but not HTLV-I tax, are likely factors in the epidermotropism of cutaneous T-cell lymphoma via induction of interferon-inducible protein-10. Leukemia and Lymphoma 29(3-4): 315-328, 1998

Antiviral and antidifferentiative activities of interferon beta and gamma in relation to their induction of double-stranded RNA-dependent protein kinase activity in 3T3-L1 cells. Journal of Biological Chemistry 263(3): 1336-1341, 1988