Effect of acyclovir on the uptake of 131I-labelled 1- (2'fluoro-2'-deoxy-beta-D-arabinofuranosyl) -5-iodouracil in herpes infected cells
Tovell, D.R.; Yacyshyn, H.P.; Misra, H.K.; Knaus, E.E.; Wiebe, L.I.; Samuel, J.; Gill, M.J.; Tyrrell, D.L.
Journal of Medical Virology 22(2): 183-188
ISSN/ISBN: 0146-6615 PMID: 3039053 DOI: 10.1002/jmv.1890220210
Selective uptake of nucleoside analogues by herpes simplex virus infected cells may serve as the basis for a specific non-invasive diagnostic test for herpes simplex encephalitis. We have examined the effect of acyclovir on the selective uptake of [131I] 1-(2'-fluoro-2'-deoxy-beta-D-arabinofuranosyl)-5-iodouracil in herpes simplex virus infected primary rabbit kidney cells. Infected cells treated with acyclovir continued to concentrate [131I] 1-(2'-fluoro-2'-deoxy-beta-D-arabinofuranosyl)-5-iodouracil for up to 24 h after the addition of the antiviral agent. These results indicated that therapy with acyclovir for as long as 24 h would not prevent the selective trapping of nucleoside analogues. This has important implications for the use of nucleoside analogues in diagnostic brain scans to detect herpes simplex encephalitis.