+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effect of cryoprotectants on protein-synthesizing activity of cell-free system from rat liver



Effect of cryoprotectants on protein-synthesizing activity of cell-free system from rat liver



Ukrains'kyi Biokhimichnyi Zhurnal 49(4): 39-42



The protein-synthetizing activity in postmitochondrial supernatant (S15) was studied as affected by ethylene glycol, PEG-400, PEG-4000, DMSO, ethanol, methanol, and glycerol. It is found that cryoprotectants inhibit 14C-leucine incorporation in the presence of physiological concentrations of Mg2+ (5 mM) in the cell-free system. All cryoprotectants, exept of glycerol, shift the maximum of 14C-leucine incorporation towards lower Mg2+ concentrations in the cell-free system.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039914846

Download citation: RISBibTeXText

PMID: 898316


Related references

Effect of cryoprotectants on protein synthesizing activity of the cell free system from rat liver. Ukrayins'kyi Biokhimichnyi Zhurnal 49(4): 39-42, 1977

Protein-synthesizing activity of membrane-bound and free polyribosomes in rat liver and sarcoma M-1 cells in a cell-free system. Doklady Akademii Nauk SSSR 229(1): 203-206, 1976

Protein synthesizing activity of membrane bound and free poly ribosomes of liver cells and sarcoma m 1 of rats under conditions of a cell free system. Doklady Biochemistry 229(1-6): (1977), 1976

Effect of fission neutron irradiation on mouse embryo 2. changes of cell free protein synthesizing system isolated from brain and liver. Acta Biochimica et Biophysica Academiae Scientiarum Hungaricae 16(3-4): 260, 1981

Effect of partial ischemia and ischemic-hypoxic hypoxia on the activity of a cell-free protein synthesizing system from rat brain. Bulletin de L'academie Polonaise des Sciences. Serie des Sciences Biologiques 20(4): 269-272, 1972

Effect of polyamines on yeast cell-free protein synthesizing system. II. Increase stability of cell-free system in the presence of spermine. Acta Microbiologica Polonica 25(3): 199-204, 1976

Effect of changes in permeability of lysosomal and mitochondrial membranes on ability of their cryoextracts to inhibit protein synthesizing activity of the cell free system. Ukrainskii Biokhimicheskii Zhurnal 87(1): 16-20, 1985

Metabolism of messenger rna of free and membrane bound poly ribosomes of rat liver in a cell free protein synthesizing system. Doklady Akademii Nauk SSSR Seriya Biologiya 217(5): 1203-1206, 1974

Synthesis of liver cytochrome P-450b in a cell-free protein synthesizing system. Biochemical and Biophysical Research Communications 91(3): 886-891, 1979

Synthesis of rat liver albumin in a rabbit reticulocyte cell free protein synthesizing system. Journal of Biological Chemistry 248(22): 7661-7668, 1973

Change in the activity of a cell-free protein-synthesizing system with different functional states of the central nervous system. Voprosy Meditsinskoi Khimii 18(2): 212-216, 1972

Translation of polysomal poly adenine containing rna of rat liver in the cell free protein synthesizing system of wheat germs. Izvestiya Sibirskogo Otdeleniya Akademii Nauk SSSR Seriya Biologicheskikh Nauk (2): 115-121, 1981

Messenger activity of sendai virus ribonucleoprotein in a cell free protein-synthesizing system. Virology 42(2): 508-521, 1970

Effect of heavy water on the cell-free protein-synthesizing system. Biochimica et Biophysica Acta 157(3): 663-665, 1968

The effect of hypophysectomy on a cell-free protein synthesizing system from rat brain. Endocrinology 90(1): 328-330, 1972