Section 40
Chapter 39,938

Effect of prostaglandin e on the adenyl cyclase-cyclic AMP system and gluconeogenesis in rat renal cortical slices

Morrison, A.R.; Yates, J.; Klahr, S.

Biochimica et Biophysica Acta 421(2): 203-209


ISSN/ISBN: 0006-3002
PMID: 175840
DOI: 10.1016/0304-4165(76)90286-5
Accession: 039937514

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Prostaglandin E was found to increase the formation of cyclic acdenosine 3',5'-monophosphate (cyclic AMP) by renal cortical slices. This increased release of cyclic AMP was not influenced by the absence of Ca2+ in the incubating media. The enhanced production of cyclic AMP was probably mediated by stimulation of membrane-bound adenylate cyclase activity. An increase in adenyl cyclase activity was observed with increasing concentrations of prostaglandin E. Furthermore, prostaglandin E augmented glucose production from alpha-ketoglutarate. This effect on gluconeogenesis was abolished by the removal of Ca2+ from the incubating medium. These effects are similar to those described for parathyroid hormone and suggest that the renal cortex is a prostaglandin-dependent system. Prostaglandin E decreased cyclic AMP production and glucose production (from alpha-ketoglutarate) in response to submaximal doses of parathyroid hormone, suggesting that prostaglandin may be important in modulating the intracelluar action of parathyroid hormone in the kidney cortex.

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