+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effect of proteolysis on the electron spin resonance spectra of maleimide spin labeled erythrocyte membrane



Effect of proteolysis on the electron spin resonance spectra of maleimide spin labeled erythrocyte membrane



Biochimica et Biophysica Acta 821(2): 175-178



The ratio of low-field amplitudes of weakly and strongly immobilized signals of ESR spectra of a maleimide spin label bound to erythrocyte membranes (hw/hs) increases progressively during incubation at 37 degrees C. This increase is due to the 'self-digestion' of membrane proteins by endogenous proteinases and is attenuated by proteinase inhibitors. Digestion of membranes with chymotrypsin also increases the hw/hs ratio. These results suggest a need for a careful interpretation of data from spin-labeled membrane proteins, especially in experiments involving prolonged incubations of membrane preparations when the proteolytic effects may be significant.

(PDF emailed within 0-6 h: $19.90)

Accession: 039937908

Download citation: RISBibTeXText

PMID: 2998467

DOI: 10.1016/0005-2736(85)90086-0


Related references

Reappraisal of the electron spin resonance spectra of maleimide and iodoacetamide spin labels in erythrocyte ghosts. Archives of Biochemistry and Biophysics 190(2): 611-616, 1978

The effect of divalent metal binding on the electron spin resonance spectra of spin-labeled actin. Evidence for spin-spin interactions involving manganese. II. Archives of Biochemistry and Biophysics 150(2): 792-796, 1972

Effects of phenothiazine derivatives on biological membranes drug induced changes in electron spin resonance spectra from spin labeled erythrocyte ghost membranes. Journal of Pharmacology and Experimental Therapeutics 173(1): 78-84, 1970

Simulation of electron spin resonance spectra of spin-labeled fatty acids covalently attached to the boundary of an intrinsic membrane protein. A chemical exchange model. Journal of Magnetic Resonance 48(3): 475-494, 1969

Saturation transfer electron spin resonance of Ca2(+)-ATPase covalently spin-labeled with beta-substituted vinyl ketone- and maleimide-nitroxide derivatives. Effects of segmental motion and labeling levels. Biophysical Journal 58(1): 231-241, 1990

The effects of nucleotides and Mg 2+ on the electron spin resonance spectra of myosin spin labeled at the S 2 thiol groups. Archives of Biochemistry and Biophysics 152(2): 839-848, 1972

Endogenous proteolysis of the human erythrocyte membrane as studied by two-dimensional gel electrophoresis and electron spin resonance. International Journal of Biochemistry 21(8): 941-945, 1989

Multifrequency electron spin resonance spectra of a spin-labeled protein calculated from molecular dynamics simulations. Journal of the American Chemical Society 131(7): 2597-2605, 2009

Simulating electron spin resonance spectra of macromolecules labeled with two dipolar-coupled nitroxide spin labels from trajectories. Physical Chemistry Chemical Physics 13(28): 12785-12797, 2012

Structural and motional changes in glyceraldehyde-3-phosphate dehydrogenase upon binding to the band-3 protein of the erythrocyte membrane examined with [15N,2H]maleimide spin label and electron paramagnetic resonance. Proceedings of the National Academy of Sciences of the United States of America 78(8): 4955-4959, 1981

The effects of saikosaponins on biological membranes. 2. Changes in electron spin resonance spectra from spin-labelled erythrocyte and erythrocyte ghost membranes. Planta Medica 34(3): 287-290, 1978

Interaction of spin-labeled apocytochrome c and spin-labeled cytochrome c with negatively charged lipids studied by electron spin resonance. Biochemistry. 33(23): 7146-7156, 1994

Electron spin resonance spin label studies of the erythrocyte membrane in Duchenne muscular dystrophy. American Journal of Medical Genetics 16(2): 153-161, 1983

Spin-labeled electron spin resonance study of the lipid-containing membrane of influenza virus. Proceedings of the National Academy of Sciences of the United States of America 68(10): 2579-2583, 1971

Immobilization-induced changes in erythrocyte membrane fluidity in rabbits: a spin-label electron spin resonance study. Annals of Clinical and Laboratory Science 32(4): 399-403, 2002