+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of a chemotactic factor, N-formylmethionyl peptide, on adherence, superoxide anion generation, phagocytosis, and microtubule assembly of human polymorphonuclear leukocytes



Effects of a chemotactic factor, N-formylmethionyl peptide, on adherence, superoxide anion generation, phagocytosis, and microtubule assembly of human polymorphonuclear leukocytes



Journal of Laboratory and Clinical Medicine 93(3): 506-514



FMLP promoted microtubule assembly in PMNs at concentrations which were chemotactic for the cells. At higher concentrations than those required for chemotaxis, FMLP enhanced the adherence of PMNs to nylon glass fibers. With colchicine, PMN adherence was inhibited, but upon exposure to FMLP, PMN adherence could be restored. The effect of FMLP on PMN adherence was transitory and was no longer evident by 5 min. At concentrations similar to those employed in the adherence studies. FMLP induced the cells to briefly generate O-2, since ferricytochrome C reduction was no longer evident by 5 min. Pretreatment of the PMNs with cytochalasin B enhanced the release of O-2 by PMNs exposed to FMLP. On the other hand, there was no effect of FMLP on phagocytosis of C3-coated particles. These results suggest that FMLP induces responsive cells to develop a hyperadherent plasma membrane which is largely independent on microtubule control. Since oligopeptides similar of AMLP are formed in bacteria, it is likely that the action of N-formylated peptides is important in regulating the inflammatory response.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 039955953

Download citation: RISBibTeXText

PMID: 219119


Related references

Effects of ethanol on the chemotactic peptide-induced second messenger generation and superoxide production in polymorphonuclear leukocytes. Journal of Infectious Diseases 166(4): 854-860, 1992

Effects of a 5-lipoxygenase inhibitor, AA861, on lipoxygenase metabolism and superoxide anion generation by human polymorphonuclear leukocytes--potentiation of superoxide anion generation by LTB4. International Archives of Allergy and Immunology 98(4): 361-369, 1992

Modulation of the inflammatory response by products released from human polymorphonuclear leukocytes during phagocytosis : Generation and inactivation of the chemotactic factor C5a. Inflammation 1(1): 23-39, 1975

Increased production of superoxide anion by neonatal polymorphonuclear leukocytes stimulated with a chemotactic peptide. American Journal of Hematology 27(3): 169-173, 1988

Inhibitory effects of N-acetylcysteine on superoxide anion generation in human polymorphonuclear leukocytes. Journal Of Pharmacy & Pharmacology. 49(5): 525-529, 1997

Signal transduction pathway in human polymorphonuclear leukocytes for chemotaxis induced by a chemotactic factor. Distinct from the pathway for superoxide anion production. Journal of Immunology 152(12): 5922-5929, 1994

Enhancing and inhibitory effects of nitric oxide on superoxide anion generation in human polymorphonuclear leukocytes. British Journal Of Pharmacology. 115(7): 1302-1306, 1995

Effects of Antiasthma Drugs on Superoxide Anion Generation from Human Polymorphonuclear Leukocytes or Hypoxanthine-Xanthine Oxidase System. International Archives of Allergy and Immunology 96(2): 128-133, 1991

Effect of immunoglobulin and complement on phagocytosis and the release of superoxide anion by human polymorphonuclear leukocytes. Nihon Ketsueki Gakkai Zasshi 46(3): 671-676, 1983

Inhibitory effects of metallothionein in superoxide generation and chemotactic activity of polymorphonuclear leukocytes. Yagi, K, Kondo, M, Niki, E, Yoshikawa, T International Congress Series; Oxygen radicals: 715-718, 1992

Eosinophil chemotactic factor (ECF). II. Release from human polymorphonuclear leukocytes during phagocytosis. Journal of Immunology 117(1): 235-241, 1976

Effects of indomethacin, 5,8,11,14-eicosatetraynoic acid, and p-bromophenacyl bromide on lysosomal enzyme release and superoxide anion generation by human polymorphonuclear leukocytes. Biochemical Pharmacology 29(4): 533-538, 1980

Superoxide anion generation of human polymorphonuclear leukocytes in urine. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 65(6): 718-724, 1991

Effect of the chemotactic peptide on the subsequent superoxide releasing response in human polymorphonuclear leukocytes. Febs Letters 128(1): 5-8, 1981

Effects of Ethanol on the Chemotactic Peptide-Induced Second MessengerGeneration and Superoxide Production in Polymorphonuclear Leukocytes. Journal of Infectious Diseases 166(4): 854-860, 1992