EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Effects of cell wall deficiency on the synthesis of polysaccharide-degrading exoenzymes: a study on mycelial and wall-less phenotypes of the fz; sg; os-1 ('slime') triple mutant of Neurospora crassa


Effects of cell wall deficiency on the synthesis of polysaccharide-degrading exoenzymes: a study on mycelial and wall-less phenotypes of the fz; sg; os-1 ('slime') triple mutant of Neurospora crassa



Journal of General Microbiology 136(8): 1463-1468



ISSN/ISBN: 0022-1287

PMID: 2262786

DOI: 10.1099/00221287-136-8-1463

The production of exoenzymes which degrade cellulose, polygalacturonic acid and xylan was studied in mycelial and wall-less phenotypic derivatives of Neurospora crassa obtained by vegetative selection applied to a single fz;sg;os-1 ('slime'-like) segregant (strain RCP-3) of a cross 'slime' x wild type. The unrelated stable 'slime' strain FGSC 1118 was also studied. The synthesis of polysaccharide-degrading enzymes was normally induced by polysaccharidic substrates and was sensitive to carbon-catabolite repression for both mycelium-forming phenotypes (mycelial intermediate and spheroplast-hyphal intermediate) of strain RCP-3. The stable 'slime' from RCP-3 produced cellulose-degrading activity and xylan-degrading activity constitutively but was fully sensitive to glucose repression. The stable 'slime' RCP-3 did not synthesize polygalacturonic-acid-degrading activity, even in the presence of inducers. For the stable 'slime' FGSC 1118, all of the polysaccharide-degrading activities were produced constitutively and were markedly resistant to glucose repression. The possible epigenetic origin of the different properties of stable 'slimes' RCP-3 and FGSC 1118 is considered. These results may relate to the role of the cell surface in the processing of regulatory signals which control the adaptation of the fungal cell to the nutritional environment.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 039961908

Download citation: RISBibTeXText

Related references

Effects of cell wall deficiency in slime strains of neurospora crassa a study on mycelial and wall less phenotypic derivatives of a single fz sg os 1 slime like segregant. Journal of General Microbiology 136(1): 121-130, 1990

Effect of cell wall deficiency of 'slime' strains of Neurospora crassa: a study on mycelial and wall-less phenotypic derivatives of a single fz; sg; os-1 ('slime'-like) segregant. The Journal of General Microbiology 136: 1-9, 1990

(1,3)-beta-D-glucan synthase activity in mycelial and cell wall-less phenotypes of the fz, sg, os-1 ("slime") mutant strain of Neurospora crassa. Experimental Mycology 19(1): 35-47, 1995

Cell wall deficiency in "slime" strains of Neurospora crassa: osmotic inhibition of cell wall synthesis and beta-D-glucan synthase activity. Brazilian Journal of Medical and Biological Research 27(12): 2843-2857, 1994

Ultrastructural and morphological characterization of slime x a neurospora crassa cell wall mutant. Abstracts of the Annual Meeting of the American Society for Microbiology 77: 185, 1977

Production of plant cell wall degrading enzymes by the exo-1 mutant and wild type Neurospora crassa. Journal of Biotechnology 150: 513-514, 2010

Chitosomes from the wall-less "slime" mutant of Neurospora crassa. Archives of Microbiology 139(2-3): 105-112, 1984

Membrane lipid composition and invertase secretion of Neurospora crassa and its wall-less mutant slime: Effects of temperature and the surfactant Tween 80. Journal of General Microbiology 139(8): 1885-1889, 1993

slime, the wall-less variant of Neurospora crassa: The loss of cell-wall assembly is not due to the loss of cell-wall primer or template. Experimental Mycology 6(3): 203-209, 1982

Studies on cell wall polysaccharides from Neurospora crassa. Part II. Change of the structure of cell wall .BETA.-1,3-D-glucan with the growth of Neurospora crassa cells. Agricultural and Biological Chemistry 48(4): 1041-1047, 1984

Studies on the cell wall of Neurospora crassa. Part V Purification and some properties of two wall-associated (.BETA.1,3)glucanases from Neurospora crassa cells. Agricultural and Biological Chemistry 50(10): 2461-2467, 1986

Cell wall polysaccharide synthesis in vitro catalyzed by an enzyme from slime mold myxamoebae lacking a cell wall. Biochemical and Biophysical Research Communications 22(3): 352-356, 1966

The Neurospora crassa CPS-1 polysaccharide synthase functions in cell wall biosynthesis. Fungal Genetics and Biology: Fg and B 69: 23-30, 2014

Copper accumulation in the cell-wall-deficient slime variant of Neurospora crassa. Comparison with a wild-type strain. Biochemical Journal 245(2): 479-484, 1987

Studies on the application of plant cell wall degrading enzymes from aspergillus terreus and neurospora crassa. Biotechnology Letters 5(7): 481-486, 1983