+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells

Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells

Molecular and Cellular Biology 6(2): 703-706

The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 039993662

Download citation: RISBibTeXText

PMID: 3466022

Related references

Stable expression of selectable genes introduced into human hematopoietic stem cells by electric field-mediated DNA transfer. Proceedings of the National Academy of Sciences of the United States of America 83(10): 3496-3499, 1986

Electric field-mediated gene transfer: characterization of Dna transfer and patterns of integration in lymphoid cells. Nucleic Acids Research 16(12): 5515-5532, 1988

Electric field-mediated gene transfer (electroporation) into mouse Friend and human K562 erythroleukemic cells. Gene Analysis Techniques 4(3): 50-56, 1987

Electric field mediated gene transfer characterization of dna transfer and patterns of integration in lymphoid genes. Nucleic Acids Research 16(12): 5515-5532, 1988

Electric field mediated gene transfer into k562 human leukemia cells. Australian & New Zealand Journal of Medicine 18(3 SUPPL 2): 437, 1988

Stable and transient expression of my 1 a human granulocytic differentiation antigen in mouse cells after transfer from acute myeloid leukemia blasts. Proceedings of the American Association for Cancer Research & American Society of Clinical Oncology 22: 154, 1981

Stable expression of the wild-type p53 gene in human lung cancer cells after retrovirus-mediated gene transfer. Human Gene Therapy 4(5): 617-624, 1993

Stable expression of a human multidrug resistance adenosine deaminase fusion gene after dna mediated transfer into human kb cells. Journal of Cell Biology 107(6 PART 3): 326A, 1988

Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer. Stem Cells 25(11): 2919-2927, 2007

Expression of the human beta globin gene and 5'-deletion mutants in erythroleukemic mouse cells studied by DNA mediated gene transfer. Anticancer Research 3(6): 409-416, 1983

In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression. Methods 33(2): 126-135, 2004

Retroviral mediated transfer and expression of the human glucose 6-phosphate dehydrogenase gene in mouse bone marrow cells. Blood 90(10 SUPPL 1 PART 1): 119A, Nov 15, 1997

Long-term stable expression of human apolipoprotein A-I mediated by helper-dependent adenovirus gene transfer inhibits atherosclerosis progression and remodels atherosclerotic plaques in a mouse model of familial hypercholesterolemia. Circulation 107(21): 2726-2732, 2003

Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal. 66(2 Part 1): 524-531, 1994

Maintenance of hypomethylation status and preferential expression of exogenous human MDR1/PGY1 gene in mouse L cells by YAC mediated transfer. Somatic Cell and Molecular Genetics 23(4): 259-274, 1997