+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. III. Retention and selectivity of proteins and peptides in gradient elution on non-porous monodisperse 1.5-microns reversed-phase silicas



Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. III. Retention and selectivity of proteins and peptides in gradient elution on non-porous monodisperse 1.5-microns reversed-phase silicas



Journal of Chromatography 397: 71-80



Following previous studies of the use of non-porous monodisperse 1.5-microns n-octyl- and n-octadecyl-bonded silicas in gradient elution of proteins, this work was aimed at elucidating further the properties of this novel column material for peptide and protein separations in comparison with wide-pore silicas. First, it is demonstrated that with short columns (e.g., 35 X 8 mm I.D.) packed with these non-porous reversed-phase materials, mixtures of small peptides and mixtures of proteins can be very efficiently resolved. When the chain length of the bonded ligand was varied, the retention of a test set of proteins in gradient elution followed the ligand sequence C18 greater than C8 approximately C4 approximately phenyl greater than C2 under constant elution conditions, and the selectivity remained unchanged. Comparison of the S values of these proteins, as determined from evaluation of the log k' vs. phi dependences with non-porous silicas and with a LiChrospher Si 1000 C8 with identical accessible ligand surface areas per unit column volume, indicated lower values for the non-porous materials (k' = capacity factor; phi = molar fraction of organic solvent; S = slope of the plot of log k' vs. phi). The origin of this behaviour is discussed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040063296

Download citation: RISBibTeXText

PMID: 2821038


Related references

Evaluation of advanced silica packings for the separation of biopolymers by high performance liquid chromatography iii. retention and selectivity of proteins and peptides in gradient elution on non porous monodisperse 1.5 mum reversed phase silicas. Journal of Chromatography 397: 71-80, 1987

Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. IV. Mobile phase and surface-mediated effects on recovery of native proteins in gradient elution on non-porous, monodisperse 1.5-microns reversed-phase silicas. Journal of Chromatography 397: 81-89, 1987

Evaluation of advanced silica packings for the separation of biopolymers by high performance liquid chromatography iv. mobile phase and surface mediated effects on recovery of native proteins in gradient elution on non porous monodisperse 1.5 mum reversed phase silicas. Journal of Chromatography 397: 81-90, 1987

Evaluation of advanced silica packings for the separation of biopolymers by high performance liquid chromatography ii. performance of non porous monodisperse 1.5 micrometer silica beads in the separation of proteins by reversed phase gradient elution high performance liquid chromatography. Journal of Chromatography 359: 61-72, 1986

Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. V. Performance of non-porous monodisperse 1.5-microns bonded silicas in the separation of proteins by hydrophobic-interaction chromatography. Journal of Chromatography 397: 91-97, 1987

Evaluation of advanced silica packings for the separation of biopolymers by high performance liquid chromatography v. performance of non porous monodisperse 1.5 mum bonded silicas in the separation of proteins by hydrophobic interaction chromatography. Journal of Chromatography 397: 91-98, 1987

Evaluation of advanced silica packings for the separation of biopolymers by high performance liquid chromatography 1. design and properties of parent silicas. Journal of Chromatography 296: 3-14, 1984

High performance liquid chromatography of amino acids peptides and proteins lxix. evaluation of retention and bandwidth relationships of myosin related peptides separated by gradient elution reversed phase high performance liquid chromatography. Journal of Chromatography 392: 33-50, 1987

High performance liquid chromatography of amino acids peptides and proteins lxviii. evaluation of retention and bandwidth relationships of peptides related to lhrh and growth hormone releasing factor separated by gradient elution reversed phase high performance liquid chromatography. Journal of Chromatography 359: 31-54, 1986

Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. VI. Design, chromatographic performance and application of non-porous silica-based anion exchangers. Journal of Chromatography 476: 37-48, 1989

High-performance liquid chromatography of amino acids, peptides and proteins. LXVII. Evaluation of bandwidth relationships of peptides related to human beta-endorphin, separated by gradient-elution reversed-phase high-performance liquid chromatography. Journal of Chromatography 352: 35-66, 1986

High-performance liquid chromatography of amino acids, peptides and proteins. XLVIII. Retention behaviour of tryptic peptides of human growth hormone isolated by reversed-phase high-performance liquid chromatography: a comparative study using different chromatographic conditions and predicted elution behaviour based on retention coefficients. Journal of Chromatography 266: 89-103, 1983

Retention mechanism, isocratic and gradient-elution separation and characterization of (co)polymers in normal-phase and reversed-phase high-performance liquid chromatography. Journal of Chromatography. a 869(1-2): 65-84, 2000

Optimization model for the gradient elution separation of peptide mixtures by reversed phase high performance liquid chromatography verification of retention relationships. Journal of Chromatography 296: 31-60, 1984

High performance liquid chromatography of amino acids peptides and proteins 53. evaluation of the effect of several stationary phase parameters on the chromatographic separation of poly peptides on alkyl silicas. Journal of Chromatography 282: 541-560, 1983