+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up



Evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up



Clinical Infectious Diseases 15(3): 473-480



The ELISA with use of IgG, IgA, and IgE, latex agglutination, indirect hemagglutination (IHA), total IgE, the radioallergosorbent test (RAST), and immunoelectrophoresis (IEP) were carried out to determine the preoperative diagnosis of infection due to Echinococcus granulosus in 131 patients. Eighty-nine patients received follow-up care for 42 +/- 22 months (mean +/- SD); 72 were treated surgically and 17 with mebendazole only. We analyzed 5 +/- 2 serum samples per patient and analyzed each serum sample using the eight tests. IgG ELISA was the most sensitive (up to 94%) and specific (up to 99%) test for the majority of cyst locations in the patient. IEP was positive in only 73% of cases. The combination of IgG ELISA, IHA, and IgA ELISA allowed us to achieve a sensitivity of 81% in cases of pulmonary echinococcosis. IgE and IgA were both responsible for most nonspecific reactions, the former in patients with parasitic diseases other than echinococcosis and the latter in patients with cirrhosis of the liver and malignancies. IgG ELISA and IHA were the most adequate tests for postsurgical follow-up. In patients with favorable clinical outcome, the specific IgG level decreased early toward the end of the first year, although serological positivity could persist beyond 6 years. A rise of IgG level 2 or 3 years after treatment suggested persistent active infection. In patients with mebendazole-associated cure, only tests with total or specific IgE detection seemed to exhibit a strong association with decreasing levels or with negative results.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040065508

Download citation: RISBibTeXText

PMID: 1520796

DOI: 10.1093/clind/15.3.473


Related references

Serological Diagnosis and Follow-Up of Human Cystic Echinococcosis: A New Hope for the Future?. Biomed Research International 2015: 428205, 2015

Evaluation of the diagnostic value of the ELISA tests developed by using EgHF, Em2 and EmII/3-10 antigens in the serological diagnosis of alveolar echinococcosis. Mikrobiyoloji Bulteni 48(3): 461-468, 2014

Evaluation of two immunoblot tests with goat and sheep hydatid antigen for human echinococcosis diagnosis. Revista Peruana de Medicina Experimental Y Salud Publica 27(2): 209-214, 2010

Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France. Parasite 21: 34, 2014

Evaluation of serological tests used in the diagnosis of human brucellosis. 1986

Evaluation of a commercial Echinococcus Western Blot assay for serological follow-up of patients with alveolar echinococcosis. Clinical and Vaccine Immunology 15(11): 1633-1637, 2008

Human echinococcosis in Poland in 2003-2010 according to the serological tests results of NIPH-NIH. Przeglad Epidemiologiczny 65(3): 455-458, 2012

Evaluation of two serological tests in diagnosis of human cases of biliary and ectopic fascioliasis. Journal of the Egyptian Society of Parasitology 32(1): 79-90, 2002

Latent class evaluation of three serological tests for the diagnosis of human brucellosis in Bangladesh. Tropical Medicine and Health 44: 32, 2016

The intravital serological diagnosis of the human echinococcosis. Third International Symposium of the Helminthological Institute, Kosice, 12-15 October, 1976, High Tatra Mountains, Czechoslovakia: [1]p., 1976

Evaluation and comparison of fluorescence polarization assay with three of the currently used serological tests in diagnosis of human brucellosis. European Journal of Clinical Microbiology and Infectious Diseases 26(10): 715-721, 2007

Diagnosis and follow-up of syphilis by serological tests. Annales de Dermatologie et de Venereologie 107(3): 121-126, 1980

Human malaria: evaluation of serological tests in seroepidemiological surveys, individual diagnosis and for blood banks screening. Memorias do Instituto Oswaldo Cruz 83(Suppl. 1): 476-477, 1988

The evaluation of the chance cross reactivity of serological tests for the diagnosis of human immunodeficiency virus and Trypanosoma cruzi infections. Revista Da Sociedade Brasileira de Medicina Tropical 25(1): 77-78, 1992

Evaluation of the radial immunodiffusion test with native hapten antigen in the serological diagnosis of brucellosis in infected and vaccinated cattle. Comparison with other serological tests. Repositorio de Trabalhos do LNIV 21: 11-39, 1989