+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library

Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library

Genomics 13(3): 873-876

Expressed sequence tags (ESTs) provide useful molecular landmarks for physical mapping and identify the position of an expressed region in the genome. The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of ESTs should reduce the repetitive isolation of constitutively expressed sequences. We report here the sequence tags from the 3'-end region of 58 new directionally cloned cDNAs from a subtracted human retinal pigment epithelium (RPE) cell line library. Eight of the cDNAs have been assigned to human chromosomes using PCR-based EST assays. Chromosomal mapping of subtracted RPE cDNA clones may also help in identifying candidate genes for inherited eye diseases.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040105553

Download citation: RISBibTeXText

PMID: 1639417

DOI: 10.1016/0888-7543(92)90173-P

Related references

Retina and retinal pigment epithelium expressed sequence tags and genes for cDNA microarrays. IOVS 41(4): S871, March 15, 2000

Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library. Genome Research 5(4): 359-367, 1995

Expression and chromosomal localization of cDNA clones from an enriched human retinal pigment epithelial (RPE) cell line library: identification of two RPE-specific genes. Cytogenetics and Cell Genetics 69(1-2): 71-74, 1995

Characterization and mapping of expressed sequence tags isolated from a subtracted cDNA library of Litopenaeus vannamei injected with white spot syndrome virus. Journal of Shellfish Research 26(4): 1247-1258, 2007

Generation of expressed sequence tags from 21 randomly selected cDNA clones derived from a bovine ovary cDNA library. Animal Genetics 27(SUPPL 2): 94, 1996

Sequencing of cDNA clones randomly chosen from an immature flower cDNA library of Chinese cabbage: expressed sequence tags. RDA Journal of Agricultural Science, Biotechnology 37(1): 167-171, 1995

Chromosomal assignment and tissue distribution of novel expressed sequence tags from a human pancreatic islet cDNA library. Genomics 29(1): 276-281, 1995

Analysis of expressed sequence tags of retinal pigment epithelium: Cystatin C is an abundant transcript. International Journal of Biochemistry & Cell Biology 32(4): 417-426, April, 2000

Sequence analysis, expression and chromosomal localization of a gene, isolated from a subtracted human retina cDNA library, that encodes an insulin-like growth factor binding protein (IGFBP2). Experimental Eye Research 52(5): 549-561, 1991

Chromosomal localization of cDNA clones from subtracted eye libraries. American Journal of Human Genetics 51(4 SUPPL ): A250, 1992

Expression and chromosomal localization in genes expressed in the retina and retinal pigment epithelium. IOVS 39(4): S915, March 15, 1998

Isolation and characterization of expressed sequence tags (ESTs) from subtracted cDNA libraries of Pennisetum glaucum seedlings. Plant Molecular Biology 64(6): 713-732, 2007

Analysis of expressed sequence tags from subtracted and unsubtracted Ctenocephalides felis hindgut and Malpighian tubule cDNA libraries. Insect molecular biology 11(4): 299-306, 2002