+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Expression of the late gene of simian virus 40 under the control of the simian virus 40 early-region promoter in monkey and mouse cells



Expression of the late gene of simian virus 40 under the control of the simian virus 40 early-region promoter in monkey and mouse cells



Journal of Virology 57(1): 91-100



We constructed a recombinant plasmid (pVNR4) with the simian virus 40 (SV40) early promoter positioned 30 nucleotides upstream from the major SV40 late transcription initiation site at residue 325. After transfection of the recombinant plasmid DNA into COS and mouse L cells, the transcripts of the SV40 late region were analyzed by S1 nuclease and primer extension analysis. The following are the principal findings. (i) The 16S and 19S late RNAs used the characteristic wild-type splice; no detectable levels of 19S unspliced RNA were observed. (ii) The majority of the late RNAs were heterogeneous and initiated in the early region (upstream and downstream from the Hogness-Goldberg sequence), and a minor population initiated at residue 325, the principal 5' terminus of the wild-type late RNA. (iii) During SV40 lytic infection there was a shift in initiation sites used to transcribe the early region from sites that are downstream to sites which are upstream (up RNA) of the origin of DNA replication. We observed that unlike lytic infection, T antigen and viral DNA replication were not needed for the appearance of up RNA in mouse L cells. (iv) In mouse L cells late RNAs were made, and the residue 325 5' end was utilized in the absence of T antigen or DNA replication. (v) In COS cells we found down RNA and up RNA transcribed from the extrachromosomally replicating plasmid but only down RNA produced by the integrated SV40 genome.

(PDF emailed within 1 workday: $29.90)

Accession: 040110140

Download citation: RISBibTeXText

PMID: 3001368


Related references

Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence. Journal of Virology 50(1): 163-173, 1984

A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter. Gene 70(1): 51-56, 1988

Control of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus. Journal of Virology 13(2): 322-330, 1974

Control of simian virus 40 gene expression at the levels of RNA synthesis and processing: thermally induced changes in the ratio of the simian virus 40 early mRNA's and proteins. Journal of Virology 35(1): 157-164, 1980

Regulation mechanism of simian virus 40 late gene expression in primary kidney cells and simian virus 40 transformed 3T3 cells. Virology 65(2): 591-594, 1975

Identification of the simian virus 40 which replicates when simian virus 40-transformed human cells are fused with simian virus 40-transformed mouse cells or superinfected with simian virus 40 deoxyribonucleic acid. Journal of Virology 6(1): 69-77, 1970

Definition of the simian virus 40 early promoter region and demonstration of a host range bias in the enhancement effect of the simian virus 40 72-base-pair repeat. Proceedings of the National Academy of Sciences of the United States of America 80(3): 721-725, 1983

Stimulation of simian virus 40 late gene expression by simian virus 40 tumor antigen. Proceedings of the National Academy of Sciences of the United States of America 81(7): 2040-2044, 1984

Evidence of transcription from the late region of the integrated simian virus 40 genome in transformed cells: location of the 5' ends of late transcripts in cells abortively infected and in cells transformed by simian virus 40. Journal of Virology 46(3): 756-767, 1983

Epidemiologic studies of latent virus infections in captive monkeys and baboons i overall plans and virus isolations with special reference to simian virus 40 and foamy viruses rhesus monkey green monkey simian virus 5. American Journal Of Epidemiology: 464-471, 1969

Specific stimulation of simian virus 40 late transcription in vitro by a cellular factor binding the simian virus 40 21-base-pair repeat promoter element. Proceedings of the National Academy of Sciences of the United States of America 84(17): 6025-6029, 1987

Evidence for simian virus 40 late transcriptional control: mixed infections of wild-type simian virus 40 and a late leader deletion mutant exhibit trans effects on late viral RNA synthesis. Journal of Virology 42(3): 798-803, 1982

Simian virus 40 DNA segment of the adenovirus 7-simian virus 40 hybrid, e46, and its transcription during permissive infection of monkey kidney cells. Journal of Virology 15(5): 1214-1221, 1975

The emergence of simian virus 40 variants in a persistent infection of rhesus monkey kidney cells and their interaction with standard simian virus 40. Virology 95(2): 598-603, 1979

5-Bromodeoxyuridine incorporation into simian virus 40 deoxyribonucleic acid. Effects on simian virus 40 replication in monkey cells. Virology 55(2): 329-338, 1973