+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Extracellular adenosine triphosphate activates calcium mobilization in human phagocytic leukocytes and neutrophil/monocyte progenitor cells



Extracellular adenosine triphosphate activates calcium mobilization in human phagocytic leukocytes and neutrophil/monocyte progenitor cells



Journal of Clinical Investigation 83(5): 1651-1660



We have examined the ability of extracellular ATP to elicit intracellular Ca2+ mobilization in a broad range of human leukocytes at particular stages of hematopoietic differentiation. The average cytosolic [Ca2+] in various leukocyte populations was measured in Fura 2-loaded cell suspensions while the cytosolic [Ca2+] in individual, Indo 1-loaded leukocytes was assayed by flow cytometric methods. Utilizing normal blood- and marrow-derived cells, human leukemic cell lines, and mononuclear cell fractions derived from the blood of patients with various leukemias, we have found that ATP-induced Ca2+ mobilization appears restricted to leukocytes of neutrophil/monocyte ontogeny. Significant ATP-induced increases in cytosolic [Ca2+] were observed in neutrophils, monocytes, and myeloid progenitor cells as immature as myeloblasts, but not in lymphocytes. Extensive characterization of the ATP-induced changes in [Ca2+] observed in the HL-60 promyelocytic cell line have indicated these Ca2+-mobilizing effects of ATP can be correlated with an activation of inositol phospholipid breakdown via the occupation of P2-purinergic receptors Significantly, of the various agonists (FMLP, platelet-activating factor, LTB4, and ATP) which elicit equivalent and maximal Ca2+ mobilization in mature neutrophils and monocytes, ATP was the most efficacious stimulant of Ca2+ mobilization in immature neutrophil/monocyte precursors. Thus, expression of putative P2-purinergic receptors for ATP appears to precede expression of other receptor types known to activate the inositol phospholipid signaling cascades in terminally differentiated phagocytes.

(PDF emailed within 1 workday: $29.90)

Accession: 040113447

Download citation: RISBibTeXText

PMID: 2708526


Related references

Extracellular adenosine triphosphate activates phospholipase C and mobilizes intracellular calcium in primary cultures of sheep anterior pituitary cells. Febs Letters 243(2): 333-336, 1989

Lysis of human monocytic leukemia cells by extracellular adenosine triphosphate: mechanism and characterization of the adenosine triphosphate receptor. Blood 82(5): 1578-1585, 1993

Activation of the inositol phospholipid signaling system by receptors for extracellular ATP in human neutrophils, monocytes, and neutrophil/monocyte progenitor cells. Annals of the New York Academy of Sciences 551: 218-37; Discussion 237-8, 1988

A calcium ion-dependent adenosine triphosphate pyrophosphohydrolase in plasma membrane from rat liver. Demonstration that the adenosine triphosphate analogues adenosine 5'-[betagamma-imido]triphosphate and adenosine 5'-[betagamma-methylene]-triphosphate are substrates for the enzyme. Biochemical Journal 171(3): 817-820, 1978

The effect of adenosine 5'-triphosphate on calcium mobilization and cell proliferation in cervical cancer cells. European Journal of Obstetrics, Gynecology, and Reproductive Biology 127(1): 110-114, 2006

An adenosine triphosphate-dependent calcium uptake pump in human neutrophil lysosomes. Journal of Clinical Investigation 76(1): 303-310, 1985

Extracellular atp activates polyphosphoinositide breakdown and calcium mobilization in ehrlich ascites tumor cells. Archives of Biochemistry & Biophysics 245(1): 84-95, 1986

Stimulation of intracellular calcium concentration by adenosine triphosphate and uridine 5'-triphosphate in human term placental cells: evidence for purinergic receptors. Journal of Clinical Endocrinology and Metabolism 80(6): 1809-1815, 1995

Intercellular calcium signaling induced by extracellular adenosine 5'-triphosphate and mechanical stimulation in airway epithelial cells. Journal of Cell Science 106: 995-1004, 1993

Adenosine triphosphate activates mitogen-activated protein kinase in human granulosa-luteal cells. Endocrinology 142(4): 1554-1560, 2001

Studies On Myofibrillar Adenosine Triphosphatase With Calcium-Free Adenosine Triphosphate. I. The Effect Of Ethylenediaminetetraacetate, Calcium, Magnesium, And Adenosine Triphosphate. Journal of Biological Chemistry 238: 3648-3653, 1963

Studies and myofibrillar adenosine tri-phosphatase with calcium-free adenosine triphosphate. I. The effect of ethylenediaminetraacetate, calcium, magnesium, and adenosine triphosphate. Jour Biol Chem 238(11): 3648-3653, 1963

Desnitro-imidacloprid activates the extracellular signal-regulated kinase cascade via the nicotinic receptor and intracellular calcium mobilization in N1E-115 cells. Toxicology & Applied Pharmacology 184(3): 180-186, November 1, 2002

Pharmacological characterization of the effect of extracellular adenosine 5-triphosphate on the concentration of free cytoplasmic calcium in rat pheochromocytoma PC12 cells. Society for Neuroscience Abstracts 20(1-2): 1548, 1994

Adenosine triphosphate activates the phospholipase-C cascade system in human amnion cells without increasing prostaglandin production. Endocrinology 124(4): 2005-2012, 1989