+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells from in vitro fertilization cycles: relationships to oocyte maturity and fertilizability and to follicular fluid steroids



Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells from in vitro fertilization cycles: relationships to oocyte maturity and fertilizability and to follicular fluid steroids



Journal of Clinical Endocrinology and Metabolism 65(4): 602-605



We measured the mitotic activity of granulosa cells, sex steroid concentrations in follicular fluids, and the maturity and fertilizability of oocytes from 49 follicles. Flow cytometric measurements of DNA were used to determine the percentage of cells in G0/G1, S, and G2/M phases of the cell cycle. Mitotic index was designated as the percentage of granulosa cells in S + G2/M. The progesterone concentration and the progesterone to estradiol ratio in follicular fluids were inversely correlated to mitotic index (r = -0.506; P less than 0.001, and r = -0.320; P less than 0.02, respectively). Estradiol and androstenedione levels did not correlate with the mitotic index. The mitotic index was higher in follicles with immature oocytes [25.6 +/- 2.0% (+/- SE); n = 7] than in follicles with mature oocytes (15.6 +/- 1.2%; n = 41; P less than 0.001). The mitotic index of granulosa cells was lowest in follicles with oocytes that fertilized (15.5 +/- 1.8%), higher in follicles with oocytes that remained unfertilized (18.5 +/- 1.3%; P less than 0.03), and highest in follicles with oocytes that fertilized abnormally (24.0 +/- 2.1%; P less than 0.02). Differences in maturity or fertilizability of oocytes were not associated with variations in follicular fluid progesterone concentrations. The study supports the concept that mitotic activity is decreased when granulosa cells become luteinized. During early follicular growth it is assumed that estradiol and perhaps androstenedione may be important regulators of cell division. Our findings suggest that progesterone, perhaps acting as an antiestradiol, is more important in controlling granulosa cell division of preovulatory follicles during the late follicular phase.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040153209

Download citation: RISBibTeXText

PMID: 3116026

DOI: 10.1210/jcem-65-4-602


Related references

Flow cytometric analysis of dna in human granulosa cells from in vitro fertilization cycles relationships to oocyte maturity and fertilizability and to follicular fluid steroids. Journal of Clinical Endocrinology & Metabolism 65(4): 602-605, 1987

Application of image analysis cytometry in follicular fluid cells obtained from in-vitro fertilization cycles: relationships to patient's age, oocyte maturity, fertilizability and in-vitro fertilization outcome. Human Reproduction 11(10): 2200-2207, 1996

Ovary and ovulation: Application of image analysis cytometry in fofficular fluid cells obtained from in-vitro fertilization cycles: relationships to patient's age, oocyte maturity, fertilizability and in-vitro fertilization outcome. Human Reproduction 11(10): 2200-2207, 1996

Human ovarian granulosa cells and follicular fluid indices: the relationship to oocyte maturity and fertilization in vitro. Human Reproduction 13(5): 1303-1306, 1998

Human chorionic gonadotrophin and steroid concentrations in follicular fluid: the relationship to oocyte maturity and fertilization rates in stimulated and natural in-vitro fertilization cycles. Human Reproduction 10(11): 2840-2844, 1995

The relationship between follicular fluid aspirate volume and oocyte maturity in in-vitro fertilization cycles. Human Reproduction 13(7): 1901-1906, 1998

Relationships of human oocyte maturity and cleavage to follicular fluid steroids. Journal Of In Vitro Fertilization & Embryo Transfer: 157, 1986

Relationship of human oocyte maturity fertilization and cleavage to follicular fluid prolactin and steroids. Journal of In Vitro Fertilization & Embryo Transfer 4(3): 168-172, 1987

Image cytometry DNA ploidy of human granulosa cells in follicular fluid from natural and stimulated in-vitro fertilization cycles. Human Reproduction 14(ABSTR BOOK 1): 99, 1999

Follicular fluid dehydroepiandrosterone sulfate is a credible marker of oocyte maturity and pregnancy outcome in conventional in vitro fertilization cycles. Journal of Human Reproductive Sciences 8(4): 209-213, 2016

Flow cytometric analysis of granulosa cells from follicular fluid after follicular stimulation. Human Reproduction 11(10): 2211-2214, 1996

Ovary and ovulation: Flow cytometric analysis of granulosa cells from follicular fluid after follicular stimulation. Human Reproduction 11(10): 2211-2214, 1996

Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells as a function of chronological age and ovulation induction regimen. Journal of Clinical Endocrinology and Metabolism 75(2): 636-640, 1992

Relationships between follicular fluid steroid hormone concentrations, oocyte maturity, in vitro fertilization and embryonic development in the rhesus monkey. Molecular Reproduction and Development 27(2): 145-151, 1990

Concentrations of angiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization: Association with oocyte maturity and fertilization. Fertility & Sterility 76(1): 98-101, 2001