+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Flow cytometric evaluation of proliferative activity and ploidy in myelodysplastic syndromes and acute leukemias

Flow cytometric evaluation of proliferative activity and ploidy in myelodysplastic syndromes and acute leukemias

Basic and Applied Histochemistry 30(2): 181-192

Propidium iodide (PI) DNA distribution of bone marrow (BM) cells was studied by flow cytometry (FCM) in 36 patients without hematologic or malignant disease (normal BM) and in 172 patients with anemias (36 pts), myelodysplastic syndromes (MDS) (33 pts) and acute leukemia (AL) at diagnosis (60 pts), remission (24 pts) and relapse (19 pts). White blood cells from normal male subjects were used as an external diploid reference standard (median CV = 3.8). Patients with normal BM, anemias, MDS and acute leukemia at diagnosis had tritiated thymidine labeling index (LI) and most with MDS and AL had also evaluable cytogenetics performed on the same BM sample used for FCM. In normal BM, median aliquot of cells with PI-DNA content intermediate between the diploid and the tetraploid value (2n-4n cells %) was 15.7. The ratio between the fluorescence intensity of the G0/1 peak of normal BM cells and the fluorescence intensity of the G0/1 peak of the reference standard (FI ratio) ranged from 93 to 1.05 (mean +/- 2SD). The 2n-4n cell % was higher than normal in anemias (p less than .001), lower in leukemias (p less than .001) and widely scattered in MDS. A linear correlation was found between 2n-4n cell % and LI, with 2n-4n cell % value higher than LI. The FI ratio was lower than normal in anemias (p less than .05), higher in AL with normal cytogenetics (p less than .02) and broadly scattered in MDS with normal cytogenetics. From our experience, PI-DNA-FCM is a simple and adequate method to evaluate proliferative activity in hematologic diseases. Nevertheless, caution must be taken in attributing small changes in FI ratio to aneuploidy, since they are found in anemias and in MDS and AL with normal cytogenetics, possibly due to differences in PI uptake by different cell types.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 040153438

Download citation: RISBibTeXText

PMID: 2427061

Related references

Relative contributions of enzyme cytochemistry and flow cytometric immunophenotyping to the evaluation of acute myeloid leukemias with a monocytic component and of flow cytometric immunophenotyping to the evaluation of absolute monocytoses. American Journal of Clinical Pathology 122(6): 865-874, 2004

Flow cytometric measurement of ploidy and proliferative activity of carcinomas of the oropharyngeal mucosa. Archives of Dermatological Research 273(1-2): 121-128, 1982

Flow cytometric DNA ploidy and cell proliferative activity in colorectal adenomatous polyps. Polish Journal of Pathology 49(3): 135-139, 1998

Flow cytometric analysis of dna ploidy and proliferative activity of colorectal carcinoma a preliminary report. Mie Medical Journal 36(2-3): 131-136, 1986

Flow cytometric analysis of cellular DNA in human acute nonlymphatic leukemias and dysmyelopoietic syndromes. Cytometry 2(4): 265-267, 1982

A comparative study of the ploidy and proliferative activity of cancer cells of different origin (a flow cytometric assay). Voprosy Onkologii (Stsburg). 1998; 44(1): 54-59, 1998

Flow Cytometric DNA-Ploidy and Proliferative Activity of Diethylnitrosamine-Induced Hepatocellular Carcinoma and Pulmonary Metastases in Monkeys. Hepatology. 22(3): 952-961, 1995

Increased caspase activity in myelodysplastic syndromes and secondary acute myeloid leukemias. Blood 92(10 SUPPL 1 PART 1-2): 419A, Nov 15, 1998

Infrequent alterations of the RAR alpha gene in acute myelogenous leukemias, retinoic acid-resistant acute promyelocytic leukemias, myelodysplastic syndromes, and cell lines. Blood 87(10): 4399-4403, 1996

Flow cytometric measurements of deoxyribonucleic acid ploidy and proliferative activity for evaluating malignant potential of human bladder cancers. Cancer Detection and Prevention 19(2): 165-172, 1995

CD117 in acute leukemias, chronic myeloproliferative disorders and myelodysplastic syndromes A paraffin immunohistochemical study with flow cytometry correlation. Laboratory Investigation 81(1): 173A, January, 2001

Flow cytometric analysis of peroxidase negative acute leukemias by monoclonal antibodies--II. Acute megakaryoblastic and acute pro-megakaryocytic leukemias. Leukemia Research 12(4): 279-289, 1988

DNA ploidy status and proliferative activity as markers of malignant potential in Barrett's esophagus: flow cytometric study using routinely paraffin-embedded tissue. World Journal of Surgery 24(1): 72-77, 1999