+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Fluorinated molecule as a tracer: difluoroserotonin in human platelets mapped by electron energy-loss spectroscopy

Fluorinated molecule as a tracer: difluoroserotonin in human platelets mapped by electron energy-loss spectroscopy

Science 200(4341): 537-539

The intracellular distribution of fluorine has been delineated in human platelets incubated with 4,6-difluoroserotonin, utilizing a scanning-transmission electron microscope equipped with an energy-loss spectrometer. Discrete intracellular structures corresponding in location to dense bodies contained high concentrations of fluorine. Electron energy-loss spectroscopy, which apparently can detect less than 10(-20) gram of fluorine in an area of 10 square nonometers, can thus localize fluorinated tracer molecules with biological activity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040156812

Download citation: RISBibTeXText

PMID: 644312

DOI: 10.1126/science.644312

Related references

Radiation damage of fluorinated organic compounds measured by parallel electron energy loss spectroscopy. Ultramicroscopy 48(1-2): 13-25, 1993

Allium cepa root tip assay in assessment of toxicity of magnesium oxide nanoparticles and microparticles. Journal of Environmental Sciences 66: 125-137, 2018

Evaluation of lanthanide tracer methods in the study of mammalian pulmonary parenchyma and cardiac muscle by electron energy-loss spectroscopy. Journal Of Microscopy (oxford). 174(3): 207-223, 1994

Demonstration of lanthanum in liver cells by energy-dispersive X-ray spectroscopy, electron energy loss spectroscopy and high-resolution transmission electron microscopy. Journal of Microscopy 223(Pt 2): 133-139, 2006

Two-dimensional boundary conditions and finite-size effects in angle-resolved photoelectron emission spectroscopy, low-energy electron diffraction, and high-resolution electron-energy-loss spectroscopy. Physical Review. B, Condensed Matter 37(6): 2884-2891, 1988

Auger-electron spectroscopy, electron-energy-loss spectroscopy, and x-ray photoemission spectroscopy studies of oxygen adsorption on the InP(111)-(1 x 1) surface. Physical Review. B, Condensed Matter 43(6): 4803-4808, 1991

Parallel electron energy-loss spectroscopy (PEELS) study of B in minerals; the electron energy-loss near-edge structure (ELNES) of the B K edge. American Mineralogist 80(11-12): 1132-1144, 1995

Electron microscopy and energy dispersive spectroscopy of washed human platelets following in vitro addition of gold complexes. Clinical Research 31(1): 156A, 1983

Manganese detected by electron spectroscopy imaging and electron energy loss spectroscopy in mitochondria of normal rat brain cells. Journal of Trace & Microprobe Techniques 20(4): 481-491, November, 2002

Formic acid on aluminum oxide: A comparison between inelastic-electron-tunneling spectroscopy and high-resolution electron-energy-loss spectroscopy. Physical Review. B, Condensed Matter 31(1): 42-51, 1985

Onset of oxidation of Al(111) at low temperatures: A study by electron-energy-loss spectroscopy and Auger electron spectroscopy. Physical Review. B, Condensed Matter 33(2): 1436-1439, 1986

Thickness dependence of signal background ratio of inner shell electron excitation loss in electron energy loss spectroscopy. Ultramicroscopy 13(3): 329-332, 1984

Electron energy loss spectroscopy EELS studies of storage sites of Crassostrea gigas contaminated by cadmium and copper through feeding Etude par EELS electron energy loss spectroscopy des sites de stockage du cadmium et du cuivre chez Crassostrea gigas contaminee par voie alimentaire. Bulletin de la Societe Zoologique de France Evolution et Zoologie, 1222: 101-108, 1997

Temperature dependence of electron irradiation induced mass loss and radiation damage in organic materials measured by electron energy loss spectroscopy. Biophysical Journalpart 2: 243a, 1985

Surface excitations in surface electron spectroscopies studied by reflection electron energy-loss spectroscopy and elastic peak electron spectroscopy. Analytical Sciences 26(2): 165-176, 2010