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Generation of photoemissive species by mitomycin C redox cycling in rat liver microsomes



Generation of photoemissive species by mitomycin C redox cycling in rat liver microsomes



Biochemical Pharmacology 36(10): 1617-1621



Generation of reactive oxygen species during redox cycling is thought to be involved in the chemotherapeutic action of quinone anticancer drugs. A clinically used agent which contains a quinone moiety is mitomycin C (Mit C). With isolated rat liver microsomes we detected photoemissive species during Mit C-induced redox cycling. After addition of reduced glutathione (GSH) a large increase in Mit C-induced chemiluminescence was observed. The increase of photoemission in deuterium oxide as well as greater than 90% of intensity at wavelengths greater than 610 nm suggest that singlet oxygen is a photoemissive species generated by this system. Glutathione disulfide (GSSG) accumulates during the reaction. We propose that superoxide anion radicals formed during redox cycling of Mit C react with GSH. Generation of glutathionyl radicals followed by oxygen addition then leads to the formation of photoemissive species and GSSG.

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Accession: 040203977

Download citation: RISBibTeXText

PMID: 3109425

DOI: 10.1016/0006-2952(87)90045-1


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