+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family

Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family

Molecular and Cellular Biology 5(12): 3417-3428

ISSN/ISBN: 0270-7306

PMID: 3939317

DOI: 10.1128/mcb.5.12.3417

Soybeans, Glycine max, synthesize a family of low-molecular-weight heat shock (HS) proteins in response to HS. The DNA sequences of two genes encoding 17.5- and 17.6-kilodalton HS proteins were determined. Nuclease S1 mapping of the corresponding mRNA indicated multiple start termini at the 5' end and multiple stop termini at the 3' end. These two genes were compared with two other soybean HS genes of similar size. A comparison among the 5' flanking regions encompassing the presumptive HS promoter of the soybean HS-protein genes demonstrated this region to be extremely homologous. Analysis of the DNA sequences in the 5' flanking regions of the soybean genes with the corresponding regions of Drosophila melanogaster HS-protein genes revealed striking similarity between plants and animals in the presumptive promoter structure of thermoinducible genes. Sequences related to the Drosophila HS consensus regulatory element were found 57 to 62 base pairs 5' to the start of transcription in addition to secondary HS consensus elements located further upstream. Comparative analysis of the deduced amino acid sequences of four soybean HS proteins illustrated that these proteins were greater than 90% homologous. Comparison of the amino acid sequence for soybean HS proteins with other organisms showed much lower homology (less than 20%). Hydropathy profiles for Drosophila, Xenopus, Caenorhabditis elegans, and G. max HS proteins showed a similarity of major hydrophilic and hydrophobic regions, which suggests conservation of functional domains for these proteins among widely dispersed organisms.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040204392

Download citation: RISBibTeXText

Related references

Molecular cloning and sequence analysis of cDNAs encoding cytoplasmic low molecular weight heat shock proteins in hexaploid wheat. Plant Science 92(1): 35-46, 1993

Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families. Journal of Molecular Biology 199(4): 549-557, 1988

The 14,000-molecular-weight antigen of Mycobacterium tuberculosis is related to the alpha-crystallin family of low-molecular-weight heat shock proteins. Journal of Bacteriology 174(4): 1352-1359, 1992

Heat shock genes in soybean molecular studies on a subset of genes for low molecular weight hs proteins. Journal of Cellular Biochemistry Suppl (7 Part B): 275, 1983

Expression of a Conserved Family of Cytoplasmic Low Molecular Weight Heat Shock Proteins during Heat Stress and Recovery. Plant Physiology 96(4): 1038-1047, 1991

Synthesis of low-molecular-weight heat-shock proteins in wheat embryos is regulated at the level of translation, that of low-molecular-weight heat-shock proteins at the level of transcription. Doklady Biochemistry 290(1-6): 322-325, 1987

Regulation of low molecular weight heat shock genes and function of their proteins in carrot. Journal of Cellular Biochemistry Suppl (15 Part A): 98, 1991

Molecular cloning and sequence analysis of expansins--a highly conserved, multigene family of proteins that mediate cell wall extension in plants. Proceedings of the National Academy of Sciences of the United States of America 92(20): 9245-9249, 1995

Cloning and characterization of genes encoding low molecular weight heat shock proteins from carrot. Plant Molecular Biology 16(4): 729-731, 1991

Genes for Bacillus megaterium small, acid-soluble spore proteins: cloning and nucleotide sequence of three additional genes from this multigene family. Journal of Bacteriology 165(2): 467-473, 1986

Trichomonas vaginalis: analysis of a heat-inducible member of the cytosolic heat-shock-protein 70 multigene family. Parasitology Research 86(7): 608-612, 2000

Tissue-Type-Specific Heat-Shock Response and Immunolocalization of Class I Low-Molecular-Weight Heat-Shock Proteins in Soybean. Plant Physiology 114(2): 429-438, 1997

Isolation of six low molecular weight heat shock proteins and partial characterization of heat shock protein 29 from mung bean hypocotyl. Phytochemistry 44(6): 985-989, 1997

The heat-shock response in rice seedlings: isolation and expression of cDNAs that encode class I low-molecular-weight heat-shock proteins. Plant and Cell Physiology 34(1): 165-168, 1993

Identification of a multigene family for small heat shock proteins in soybean and physical characterization of one individual gene coding region. Plant Molecular Biology 2(5): 269-278, 1983