+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein



Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein



Journal of Biological Chemistry 260(27): 14547-14555



The COOH terminus of the externally disposed variant surface glycoprotein (VSG) of the eukaryotic pathogenic protozoan Trypanosoma brucei strain 427 variant MITat 1.4 (117) is covalently linked to a novel phosphatidylinositol-containing glycolipid. This conclusion is supported by analysis of the products of nitrous acid deamination or Staphylococcus aureus phosphatidylinositol-specific phospholipase C treatment of purified membrane-form VSG. Lysis of trypanosomes is accompanied by release of soluble VSG, catalyzed by activation of an endogenous phospholipase C. The only apparent difference between membrane-form VSG and soluble VSG is the removal of sn-1,2-dimyristylglycerol. The COOH-terminal glycopeptide derived by Pronase digestion of soluble VSG was characterized by chemical modification and digestion with alkaline phosphatase. The results are consistent with the single non-N-acetylated glucosamine residue being the reducing terminus of the oligosaccharide and in a glycosidic linkage to a myo-inositol monophosphate that is probably myo-inositol 1,2-cyclic monophosphate. A partial structure for the VSG COOH-terminal moiety is presented. This structure represents a new type of eukaryotic post-translational protein modification and membrane anchor. We discuss the relevance of this structure to observations that have been made with other eukaryotic membrane proteins.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040223029

Download citation: RISBibTeXText

PMID: 4055788


Related references

Glycosyl sn 1 2 dimyristylphosphatidylinositol is the membrane anchor for trypanosoma equiperdum and trypanosoma congolense variant surface glycoproteins. Molecular & Biochemical Parasitology 24(2): 131-136, 1987

Biosynthesis of the glycosyl phosphatidylinositol anchor of Trypanosoma brucei variant surface glycoprotein. Biochemical Society Transactions 18(5): 722-724, 1990

Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane. Science 239(4841 Pt 1): 753-759, 1988

Evidence of myristylated disulfide-linked dimer of variant surface glycoprotein of Trypanosoma brucei-brucei. Comparative Biochemistry and Physiology. B Comparative Biochemistry 92(4): 705-710, 1989

Variant surface glycoprotein of Trypanosoma brucei brucei AnTat 1.1: influence of the isolation conditions upon the disulfide linked dimer/monomer ratio. Comparative Biochemistry and Physiology. B Comparative Biochemistry 95(2): 281-286, 1990

Characterization of the cross-reacting determinant (CRD) of the glycosyl-phosphatidylinositol membrane anchor of Trypanosoma brucei variant surface glycoprotein. European Journal of Biochemistry 1763: 527-534, 1988

The inactivation and reactivation of an expression-linked gene copy for a variant surface glycoprotein in Trypanosoma brucei. Embo Journal 3(6): 1345-1351, 1984

Carbohydrate is linked through ethanolamine to the C -terminal amino acid of Trypanosoma brucei variant surface glycoprotein. Biochemical Journal 209(1): 261-262, 1983

Carbohydrate is linked through ethanolamine to the C-terminal amino acid of Trypanosoma brucei variant surface glycoprotein. Biochemical Journal 209(1): 261-262, 1983

Biosynthesis of a variant surface glycoprotein of Trypanosoma brucei. Processing of the glycolipid membrane anchor and N-linked oligosaccharides. Journal of Biological Chemistry 263(33): 17697-17705, 1988

Characterization of the expression-linked gene copies of variant surface glycoprotein 118 in two independently isolated clones of Trypanosoma brucei. Nucleic Acids Research 10(7): 2353-2366, 1982

Expression of a variant surface glycoprotein of Trypanosoma gambiense in procyclic forms of Trypanosoma brucei shows that the cell type dictates the nature of the glycosylphosphatidylinositol anchor attached to the glycoprotein.. Biochemical journal5, 324(3): 885-895, 1997

Biosynthesis and processing of a variant surface glycoprotein from Trypanosoma brucei brucei. Archives of Biochemistry and Biophysics 2461: 460-468, 1986

Trypanosoma brucei brucei variant surface glycoprotein contains non- N -acetylated glucosamine. Biochemical Journal 234(2): 481-484, 1986

Trypanosoma brucei brucei variant surface glycoprotein contains non-N-acetylated glucosamine. Biochemical Journal 234(2): 481-484, 1986