Vitamin heterotrophy was studied among the following new yeast genera: Leucosporidium, Rhodosporidium and Filobasidium. The requirement in growth factors of various crossing types was compared among these genera and ascomycetens yeast cultures Saccharomycopsis lipolytica and Metschnikowia pulcherrima. Various crossing types of Filobasidium capsuligenum were found to differ in their requirement in growth factors. Various crossing types of Rhodosporidium and Saccharomycopsis lipolytica were stimulated by different growth substances. These characteristics can be used as markers while selecting the types of crossing.